Comparative genomic analysis revealed that dietary habits affected the adaptation of Bifidobacterium bifidumto the intestinal tract in different geographic populations

Min Li ¹Jie Yu ²Weicheng Li ²Qiong Wu ¹Jiaqi Sun ¹Zhihong Sun³

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作者信息

1. Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010018,China;Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China
2. Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010018,China;Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;Collaborative Innovative Center for Lactic Acid Bacteria and Fermented Dairy Products,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010018,China
3. Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010018,China;Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot 010018,China;Center for Applied Mathematics Inner Mongolia,Hohhot 010018,China
折叠

Abstract

Recent research on the genome of Bifidobacterium bifidum has mainly focused on the isolation sources(intestinal tract niche)recently,but reports on the isolation region are limited.This study analyzed the differences in the genome of B.bifidum isolated from different geographical populations by comparative genomic analysis.Results at the genome level indicated that the GC content of American isolates was significantly higher than that of Chinese and Russian isolates.The phylogenetic tree,based on 919 core genes showed that B.bifidum might be related to the geographical characteristics of isolation region.Furthermore,functional annotation analysis demonstrated that copy numbers of carbohydrate-active enzymes(CAZys)involved in the degradation of polysaccharide from plant and host sources in B.bifidum were high,and 18 CAZys showed significant differences across different geographical populations,indicating that B.bifidum had adapted to the human intestinal environment,especially in the groups with diets rich in fiber.Dietary habits were one of the main reasons for the differences of B.bifidum across different geographical populations.Additionally,B.bifidum exhibited high diversity,evident in glycoside hydrolases,the CRISPR-Cas system,and prophages.This study provides a genetic basis for further research and development of B.bifidum.

Key words

Bifidobacterium bifidum/Comparative genomics/Genetic diversity/Adaptation/Carbohydrate-active enzymes

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基金项目

National Key R&D Program of China(2022YFD21007002)

National Natural Science Foundation of China(32325040)

Inner Mongolia Science&Technology planning project(2022YFSJ0017)

earmarked fund for CARS36()

出版年

2024

食品科学与人类健康(英文)

CSTPCD

ISSN：

段落导航