γ-Aminobutyric acid alleviates litchi thaumatin-like protein-induced inflammation and reduces gut microbial translocation

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外文摘要：Previous research reported litchi thaumatin-like protein(LcTLP)could lead to inflammation,which is a factor causing the adverse reactions after excessive intake of litchi.As a main amino acid in litchi pulp,γ-aminobutyric acid(GABA)was found with anti-inflammatory effect.Therefore,this study aimed to investigate the effects of GABA on LcTLP-induced inflammation through RAW264.7 macrophages and C57BL mice models.In vitro study showed GABA could effectively regulate the level of inflammatory cytokines(interleukin(IL)-1β,IL-6,IL-10,and prostaglandin E2)and Ca2+in cells,and inhibit the phosphorylation of p65,IκB,p38,c-Jun N-terminal kinase(JNK)and extracellular signal-regulated kinase(ERK).These results indicate GABA alleviated inflammation through nuclear factor-κB and mitogen-activated protein kinase pathway signaling pathways.In vivo experiment was performed to verify the anti-inflammatory effect of GABA,and the results demonstrated that GABA reduced the inflammation and oxidative stress in the liver of LcTLP-treated mice,as it down-regulated the pro-inflammatory cytokines,malondialdehyde,aspartate transferase,and alanine transaminase.The relative expression of phosphorylated p38,JNK and ERK in mice liver with GABA treatment were reduced to 65%,39%and 80%of the control group,respectively.Furthermore,GABA treatment enriched probiotic bacteria and decreased pathogenic bacteria in mice gut,which reveals GABA could effectively reduce the translocation of gut microbiota.

外文关键词：

Anti-inflammationGut microbiotaLitchi thaumatin-like proteinMitogen-activated protein kinase pathwayγ-Aminobutyric acid

作者：

Yao Wang、Dongwei Wang、Kai Wang、Min Zhao、Cizhou Li、Yu Wang、Xuwei Liu、Lei Zhao、Zhuoyan Hu

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作者单位：

Guangdong Provincial Key Laboratory of Food Quality and Safety,College of Food Science,South China Agricultural University,Guangzhou 510642,China

Guangzhou Wanglaoji Lychee Industry Development Co.,Ltd.,Guangzhou 510623,China

基金：

China Agriculture Research System of MOF and MARAGuangzhou Wanglaoji Lychee Industry Research ProjectScience and Technology Planning Project of Guangzhou City of ChinaNational Natural Science Foundation of ChinaDongguan Key R&D Programme

项目编号：

CARS-325100-H220577202103000054322020222022120030008

出版年：

2024

DOI：

10.26599/FSHW.2022.9250251

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(5)