Comparative proteomics analysis reveals the domesticated Lepista sordida primordium differentiation regulation mechanism and the subsequent different development patterns in the pileus and stipe

扫码查看

原文链接

万方数据
维普

外文摘要：The wild Lepista sordida is a kind of precious and rare edible fungus.An excellent strain of it by artificial domestication was obtained,which was high-yield and high in iron content.In this study,high-throughput comparative proteomics was used to reveal the regulatory mechanism of its primordium differentiation in the early fruiting body formation.The mycelium before the primordium differentiation mainly expressed high levels of mitochondrial functional proteins and carbon dioxide concentration regulatory proteins.In young mushrooms,the highly expressed proteins were mainly involved in cell component generation,cell proliferation,nitrogen compound metabolism,nucleotide metabolism,glutathione metabolism,and purine metabolism.The differential regulation patterns of pileus and stipe growth to maturity were also revealed.The highly expressed proteins related to transcription,RNA splicing,the production of various organelles,DNA conformational change,nucleosome organization,protein processing,maturation and transport,and cell detoxification regulated the pileus development and maturity.The proteins related to carbohydrate and energy metabolism,large amounts of obsolete cytoplasmic parts,nutrient deprivation,and external stimuli regulated the stipe development and maturity.Multiple CAZymes regulated nutrient absorption,morphogenesis,spore production,stress response,and other life activities at different growth and development stages.

外文关键词：

Lepista sordidaComparative proteomicsPrimordial differentiationGrowth and development regulationPileusStipe

作者：

Xiuqing Yang、Meijie Liu、Yanjiao Zhang、Lizhong Guo、Junqi Wang、Sizhu Li、Yan Zhang、Xiaobo Li、Lili Xu、Hao Yu

展开 >

作者单位：

Shandong Provincial Key Laboratory of Applied Mycology,School of Life Sciences,Qingdao Agricultural University,Qingdao 266109,China

Shandong Agricultural Technology Extending Station,Jinan 250100,China

Shandong Mushroom Industrial Technology Innovation Research Institute,Jining 273500,China

基金：

Shandong Edible Fungus Agricultural Technology SystemNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaShandong Provincial Natural Science Foundation,ChinaQingdao Agricultural University Scientific Research Foundationhorizontal project:Breeding and property protection of new varieties of factory produced Hypsizygus marmoreus

项目编号：

SDAIT-07-023200004132272789ZR2020QC005663112007620183702012614

出版年：

2024

DOI：

10.26599/FSHW.2023.9250051

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(5)