Abstract
In the present study we investigated the impact of the combination of astragalin and rutin(CAR)on restoring gut-microbial dysbiosis and obesity and obesity related disorders.Randomized male C57BL/6J mice were experimentally divided into 5 groups and fed either a normal diet or a high-fat diet(HFD)for 16 weeks.Compared to vehicle treated group(HFD group),CAR could substantially improve selected gut microbiota abundance(Akkermansia,Lactobacillus,Bifidobacteria,Roseburia,Prevotella),reversed the Firmicutes/Bacteroidetes proportions,and inhibited the growth of Escherichia coli,Salmonella,and Klebsiella in obese mice.In addition,CAR-treated mice showed significantly increased total short-chain fatty acid production,reduced body weight gain,organs'weights,serum lipid profile(except HDL)and insulin resistance.The mRNA expressions of CCAAT/enhancer binding protein-α(C/EBP-α),sterol regulatory element-binding protein-1c(SREBP-1c),peroxisome proliferator-activated receptor-γ(PPAR-γ),acetyl-CoA carboxylase(ACC),adipocyte protein 2(aP2),and fatty acid synthase(FAS)were downregulated(P＜0.05)and the protein expression of PPAR-γ was downregulated while adenosine 5'monophosphate-activated protein kinase(AMPK)was phosphorylated in CAR-treated HFD-fed mice compared to the HFD control group.Interestingly,CAR-treated HFD-fed mice showed significantly improved tissue architecture in the liver and fatty tissues.In conclusion,the findings suggest that CAR/Moringa oleifera may be beneficial in the treatment of insulin resistance and obesity disorders.
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