Application of solid-phase extraction and gas chromatography-mass spectrometry(SPE-GC-MS)in resolution of metabolism pattern of higher alcohols in rat plasma

Yufei Liu ¹Xiaonian Cao ²Zhilei Zhou ³Qingxi Ren ³Zhongwei Ji ³Min Gong ⁴Yuezheng Xu ⁴Weibiao Zhou ⁵Shuguang Chen ⁶Jian Mao⁷

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作者信息

1. National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Jiangsu Provincial Engineering Research Center for Bioactive Product Processing,Jiangnan University,Wuxi 214122,China
2. National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Jiangsu Provincial Engineering Research Center for Bioactive Product Processing,Jiangnan University,Wuxi 214122,China;Luzhou Laojiao Co.,Ltd.,Luzhou 646000,China
3. National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Shaoxing Key Laboratory of Traditional Fermentation Food and Human Health,Jiangnan University(Shaoxing)Industrial Technology Research Institute,Shaoxing 312000,China
4. National Engineering Research Center for Huangjiu,Zhejiang Guyuelongshan Shaoxing Wine Co.,Ltd.,Zhejiang Shaoxing Huangjiu Industry Innovation Service Complex,Shaoxing 312000,China
5. Shaoxing Key Laboratory of Traditional Fermentation Food and Human Health,Jiangnan University(Shaoxing)Industrial Technology Research Institute,Shaoxing 312000,China;Department of Food Science and Technology,National University of Singapore,Singapore 117542,Singapore
6. Department of General Surgery,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College(CAMS & PUMC),Beijing 100730,China
7. National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China;Jiangsu Provincial Engineering Research Center for Bioactive Product Processing,Jiangnan University,Wuxi 214122,China;Shaoxing Key Laboratory of Traditional Fermentation Food and Human Health,Jiangnan University(Shaoxing)Industrial Technology Research Institute,Shaoxing 312000,China;National Engineering Research Center for Huangjiu,Zhejiang Guyuelo
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Abstract

Higher alcohols are key factors affecting sensory quality and post-drinking comfort of alcoholic beverages.A strategy combining solid-phase extraction and gas chromatography-mass spectrometry(SPE-GC-MS)was established to analyze the metabolism pattern of higher alcohols in rat plasma after gavage of 4 common alcoholic beverages including huangjiu,baijiu,wine and brandy.7 mL of dichloromethane was determined as the optimal extraction condition,and 8 higher alcohols were precisely quantified with detection limits of 1.82-11.65 pg/L,recoveries of 89.07％-110.89％and fine repeatability.The fastest absorption and elimination rates of plasma total higher alcohols were observed in baijiu and huangjiu group,respectively,and the highest peak concentration was found in brandy group.Additionally,the metabolic rate of plasma isoamyl alcohol in huangjiu group was faster than that in wine group at the same intragastric administration dosage.This study may provide potential insight for evaluation of alcoholic beverage quality.

Key words

Higher alcohols/Alcoholic beverages/SPE-GC-MS/Pharmacokinetic parameters

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出版年

2024

食品科学与人类健康(英文)

CSTPCD

ISSN：

段落导航