Dendrobium officinale flowers flavonoids enriched extract protects against acute ethanol-induced gastric ulcers via AMPK/PI3K signaling pathways

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外文摘要：Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium officinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible action mechanisms of D.officinale flowers'flavonoid enriched extract(DOFF)were assessed against gastric ulcer.The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34％of the total extractive was flavonoid,and ultra-high performance liquid chromatography time of flight mass spectrometer(UPLC-Q-TOF/MS)revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids.In addition,in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions,with drop-in ulcer index from 64.33±8.76 to 32.00±4.47.Similar results were revealed in human gastric mucosal epithelia cells,with cells viability to increase from 27.2％to 61.6％post DOFF administration.To analyzed the protect effect of DOFF,we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways.The results showed that DOFF(320 μg/mL)could increase the level of oxidation marker protein(HO-1),apoptosis regulatory protein(Bcl-2)and autophagy marker(LC3β)by 50.84％,43.85％,and 59.21％compared with ethanol-treated group respectively.Further analyzed of the mitochondrial activity and apoptosis pathway,we found that DOFF appeared to mitigate against ethanol-induced gastric mucosal injury via AMPK/mTOR/ULKl and PI3K/AKT autophagy signaling pathways.

外文关键词：

Dendrobium officinale flowerEthanolFlavonoidsGastric injuryAutophagy

作者：

Zhiyu Zhang、Hualing Xie、Mohamed A.Farag、Zhenhao Li、Qingping Wu、Ping Shao

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作者单位：

Department of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China

Pharmacognosy Department,College of Pharmacy,Cairo University,Cairo 12613,Egypt

Zhejiang ShouXianGu Botanical Drug Institute Co.,Ltd.,Hangzhou 321200,China

Guangdong Provincial Key Laboratory of Microbial Safety and Health,State Key Laboratory of Applied Microbiology Southern China,Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,China

Eco-Industrial Innovation Institute Zhejiang University of Technology,Quzhou 324000,China

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出版年：

2024

DOI：

10.26599/FSHW.2023.9250048

食品科学与人类健康(英文)

CSTPCD

ISSN：

年,卷(期)：2024.13(6)