首页|Identification and analysis of immunological activity of two isoforms of tropomyosin in Alectryonella plicatula
Identification and analysis of immunological activity of two isoforms of tropomyosin in Alectryonella plicatula
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
万方数据
Oyster,as a common aquatic food,play an important role in shellfish allergy.In this study,2 tropomyosin(TM)isoforms TM-α and TM-β(TM-α/-β)in Alectryonella plicatula were identified.The sequences of 852 bp encoding 284 amino acids of TM-α/-β and 2 recombinant proteins were obtained,respectively.There were 12 amino acid differences between TM-α/-β.The results of immunological experiments indicated that TM-β had stronger immunobinding activity and immunoreactivity than those of TM-α.Structural analysis showed that TM-β had more α-helix and higher surface hydrophobicity than TM-α.Sequences and epitopes alignment with shellfish TMs revealed that amino acids of TM-β were more frequently recognized as IgE epitopes in other shellfish TMs than TM-α.Differences in structure and sequence account for the higher immunological activity of TM-β compared to TM-α.These findings provide a theoretical basis for enriching the understanding of shellfish TM and accurate diagnosis of allergic components.
Shiqiang Yang、Yexin Chen、Fei Huan、Xinrong He、Xiao Yun、Hong Liu、Guixia Chen、Guangming Liu
展开 >
College of Ocean Food and Biological Engineering,Xiamen Key Laboratory of Marine Functional Food,Fujian Provincial Engineering Technology Research Center of Marine Functional Food,Jimei University,Xiamen 361021,China
Women and Children's Hospital Affiliated to Xiamen University,Xiamen 361003,China
College of Marine Biology,Xiamen Ocean Vocational College,Xiamen,Fujian 361100,China
万方数据
