首页|Development of a competitive array for discriminative determination of amphenicols in egg based on ribosomal protein L16
Development of a competitive array for discriminative determination of amphenicols in egg based on ribosomal protein L16
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Objective:Amphenicols(chloramphenicol,thiamphenicol and florfenicol)can cause aplastic anaemia and other severe side effects to consumers;therefore,it is necessary to inspect their residues in foods of animal origin.However,there has been no report on the use of amphenicols re-ceptor for the determination of their residues,and none of the previously reported immunoassays for amphenicols can differentiate the specific species.Materials and Methods:In this study,the ribosomal protein L16 of Escherichia coli was first expressed,and its intermolecular interaction mech-anisms with the three amphenicols was studied using the molecular docking technique.The protein was then combined with three enzyme-labelled conjugates to develop a direct competitive array on microplate for determination of the three drugs in egg.Results:Due to the use of principal component analysis to analyse the data,this method could discriminate the three drugs in the range 0.1-10 ng/mL,and the limits of detection for the three drugs were in the range of 0.0002-0.0009 ng/mL.The analysis results for the unknown egg samples were consistent with a liquid chromatography-tandem mass spectrometry method,and the method performances were superior to the previous immunoassays for amphenicols.Conclusion:This is the first paper reporting the use of ribosomal protein L16 to develop a competitive array for discriminative determination of amphenicols in food samples.
Amphenicolsribosomal protein L16recognition mechanismcompetitive arraydiscriminative determinationegg
才艺、马宁、吴宁鹏、王建平
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College of Veterinary Medicine,Hebei Agricultural University,Baoding,China
Henan Institute of Veterinary Drug and Feed Control,Zhengzhou,China
National Natural Science Foundation of ChinaNatural Science Foundation of Hebei Province,China
NETL
NSTL
万方数据
