首页|Inhibitory effect and mechanism of tannic acid against two starch digestive enzymes
Inhibitory effect and mechanism of tannic acid against two starch digestive enzymes
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Backgrounds:Tannic acid(TA),as a plant-derived phenolic substance,is involved in regulating the activity of starch digestive enzymes,but its underlying mechanism remains unclear.Methods and Results:In the present study,inhibition rate and inhibition kinetics assays were performed and confirmed that TA had a strong inhibitory effect on both α-amylase and α-glucosidase with IC50 values of 0.1585 mg/mL and 0.00542 mg/mL,respectively,through a mixed inhibition mode.The secondary structures of both enzymes were confirmed to be modified by TA through circular dichroism(CD)spectra.Fluorescence quenching analysis revealed that the interaction between TA and two enzymes was a static process of pontaneous complex for-mation.Finally,molecular docking revealed that non-covalent bonds were the main interaction forces between TA and both enzymes.Conclusions:Thus,TA was a promising candidate for the inhibition of starch-digesting enzymes,and the present research provided insight into postprandial glucose regulation through polyphenols.
Department of Food Science and Nutrition,Zhejiang Key Laboratory for Agri-Food Processing,Fuli Institute of Food Science,Zhejiang R&D Centre for Food Technology and Equipment,Zhejiang University,Hangzhou,China
Institute of Science and Technology,Moutai Group,Zunyi,China
Key Laboratory of Industrial Microbial Resources Development,Kweichow Moutai Co.,Ltd.,Renhuai,China
Institute of Science and Technology,Moutai Group,China
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