首页|Unraveling betalain suppression in pitaya:insights from co-activator HuMYB9 binding at HuCYP76AD1-1,HuADH1,and HuDODA1 super-enhancers
Unraveling betalain suppression in pitaya:insights from co-activator HuMYB9 binding at HuCYP76AD1-1,HuADH1,and HuDODA1 super-enhancers
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Objectives:Myeloblastosis(MYB)proteins,recognized as crucial transcriptional regulators,play an integral role in orchestrating the range of plant colors.Materials and Methods:Pitaya(Hylocereus spp.),a distinctive commercially cultivated fruit,stands apart due to its unique betalains,water-soluble nitrogen-enriched pigments that confer its vibrant hues.Although betalain biosynthesis has been extensively explored,the poten-tial influence of co-activators that might disrupt pigment production remains relatively unexplored.Results:In this study,we found that HuMYB9,an R2R3-MYB repressor,exhibited a remarkable decreasing during pitaya maturation.Sequence alignment analyses showed conserved R2 and R3 domains within the C-terminal of HuMYB9.HuMYB9's regulatory activities were found to be nuclear localized and it interacted with specific elements within the promoters of HuDODA1,HuADH1,and HuCYP76AD1-1,thus influencing the transcriptional activities in vitro.HuMYB9 transiently downregulated the expression of key betalain biosynthetic genes with a corresponding effect on the levels of pitaya pulp betalains.Conclusions:Our results suggest that HuMYB9 operates as a suppressor,specifically downregulating the expression of HuCYP76AD1-1,HuDODA1,and HuADH,thereby modulating betalain biosynthesis in pitaya.Collectively,our findings provide invaluable insights into the regula-tion of betalain accumulation in pitaya.
Fangfang Xie、Kamran Shah、Canbin Chen、Irfan Ali Sabir、Jiayi Chen、Jiaxuan Chen、Jianye Chen、Yonghua Qin
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Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China),Ministry of Agriculture and Rural Affairs,College of Horticulture,South China Agricultural University,Guangzhou,China
Department of Horticulture,College of Agriculture,Guangxi University,Nanning,China
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