摘要
以干燥的空心莲子草粉末为原料,采用水提醇沉法提取空心莲子草水溶性多糖并进行纯化.研究了料水比、浸提温度、滤液浓缩比、醇沉乙醇用量和浸提的时间对多糖收率的影响.最佳条件为:料水比1∶20 g/mL,浸提温度60℃,滤液浓缩比1/5,醇沉乙醇用量2.5倍体积乙醇,浸提时间0.5 h,此条件下粗糖的收率为11.6％,粗糖的纯度为34.2％,多糖的收率为4.0％.对比了活性炭和AB-8大孔树脂吸附法纯化空心莲子草粗糖的效果,AB-8大孔树脂效果最佳.用红外光谱、核磁共振谱和凝胶色谱等对多糖进行了结构表征.
Abstract
The water-soluble polysaccharide of Alternanthera philoxeroides was extracted and purified by water extraction and alcohol precipitation method with dry Alternanthera philoxeroides powder as raw material.The effects of solid-liquid ratio,extraction temperature,filtrate concentration ratio,ethanol amount,extraction time,and purification conditions on the yield of polysaccharides were studied.The results showed that the solid-liquid ratio was 1∶20 g/mL,the extraction temperature was 60℃,the filtrate concentration ratio was 1/5,the filtrate concentrate was added with 2.5 times the volume of ethanol,and the extraction time was 0.5 h.Under this condition,the yield of crude sugar was 11.6％,the purity of crude sugar was 34.2％,and the yield of polysaccharides was 4.0％.AB-8 macroporous resin is the optimal option to purify the crude sugar of Alternanthera philoxeroides.The composition,structure,and yield of various polysaccharides were analyzed by infrared spectroscopy,nuclear magnetic resonance spectroscopy,and gel chromatography.
基金项目
蚌埠学院自然科学研究项目(2022ZR02zd)
安徽省教育厅自然科学项目(KJ2020ZD65)
安徽省教育厅自然科学项目(KJ2021A1123)
安徽省教育厅自然科学项目(2022AH051920)
安徽省质量工程大学生创新创业项目(S202211305005)
安徽省质量工程大学生创新创业项目(S202211305007)
维普
万方数据