The DNA barcodes ITS1,ITS2 and their secondary structure were used to identify Morinda officinalis and its adulterants
[OBJECTIVE]The secondary structure constructed by ITS1 and ITS2 base sequences and ITS1 and ITS2 base sequences of DNA barcodes was used to identify Morinda officinalis and its adulterants.[METHODS]Codon Code Aligner 17.0 or SeqMan software is used to splice the biaxial sequence obtained by sequencing,download the ITS1 and ITS2 base sequence of M.officinalis and its blends from GenBnak,and then compare and analyze the base sequence obtained by sequencing with MEGA 11.0 software.The ITS1 and ITS2 base sequences of sequencing samples were obtained.All ITS1 and ITS2 base sequences were comparedand analyzed by MEGA 11.0 software,NJ and ML systematic cluster trees were constructed respectively,and intraspectic and interspecific genetic distances,interspecific base variation sites and reduced information sites of each species were calculated.The secondary structure of ITS1 and ITS2 base sequences of each species was constructed by using online web pages.[RESULTS]Based on ITS1 base sequence,the genetic distance between M.officinalis,Morinda cochinchinensis,Damnacanthus indicus,Morinda parvifolia,Morinda shuanghuaensis,Kadsura longipedunculata,Schisandra propinqua,Morinda umbellata,respectively were 0.034,0.233,0.052,0.052,1.004,1.004 and 0.049.The interspecific parsimony information loci were 6,30,9,9,90,90 and 10,respectively.According to the NJ system clustering tree,it could not be identified between D.indicus and M.umbellata,and S.propinqua and K.longipedunculata,while the ML system clustering tree showed that S.propinqua and K.longipedunculata could not be identified.Based on ITS2 base sequence,the interspecific genetic distance between M.officinalis,M.cochinchinensis,D.indicus,M.parvifolia,M.shuanghuaensis,K.longipedunculata,S.M.umbellata,were 0.014,0.113,0.034,0.015,0.509,0.516,and 0.017,respectively.Interspecific parsimony information loci were 7,34,15,8,91,88 and 9,respectively.According to the NJ system clustering tree,all the other species were clustered independently into a single branch except S.propinqua and K.longipedunculata.The ML system clustering tree showed that all the species were clustered independently into a single branch.Accurate identification of each species.The secondary structure built based on ITS2 can.[CONCLUSION]The ML phylogenetic tree and secondary structure constructed based on ITS2 could identify M.officinalis and its hybrid,and the species identification ability of ITS2 sequence was better than that of ITS1 sequence.
万方数据
维普
