探究参与安格斯牛皮下脂肪发育相关基因和信号通路，为安格斯牛选育提供借鉴与参考。分析、比较了出生后第Od与300 d的安格斯牛皮下脂肪组织差异表达基因(Differentially expressed genes，DEGs)，并对DEGs进行功能富集、蛋白质互作网络及Hub基因分析。结果发现，出生后第0 d与300 d安格斯牛皮下脂肪组织DEGs共164个，其中在300 d显著上调、下调的基因分别为130个和34个;功能富集表明，DEGs参与皮下脂肪发育相关的生物过程和信号通路，例如与代谢过程、生物调节、多细胞器官过程、细胞刺激反应、发育过程、代谢途径、蛋白质加工、谷胱甘肽代谢及氧化磷酸化等;Hub基因分析显示，60S核糖体蛋白L(60S ribosomal protein L，RPL)、肌钙蛋白T(Troponin T，TNNT)、肌球蛋白轻链(Myosin light chain，MYL)和核糖体蛋白 S(Ribosomal protein S，RPS)基因簇可能在安格斯牛皮下脂肪发育过程发挥重要作用。
Screening and identification of genes and signaling pathways related to the development of subcutaneous fat in Angus Cattle
The aim of this study was to investigate the genes and signaling pathways involved in the subcutaneous fat development of Angus cattle and provide insights for breeding purposes.Differentially expressed genes(DEGs)were analyzed between adipose tissues of Angus cattle at 0 and 300 days postnatal,and these DEGs were further utilized for functional enrichment,protein interaction network construction,and identification of Hub genes.The findings revealed 164 DEGs between subcutaneous adipose tissues of Angus cattle at 0 and 300 days after birth,with 130 genes being significantly up-regulated and 34 genes down-regulated at 300 days after birth.Functional enrichment analysis indicated the involvement of DEGs in various biological processes and signaling pathways associated with adipogenesis,such as metabolic processes,biological regulation,multi-cellular organ processes,cellular stimulation responses,developmental processes,metabolic pathways,protein processing,glutathione metabolism,and oxidative phosphorylation.Hub gene analysis identified 60S ribosomal protein L(RPL),Troponin T(TNNT),and Myosin light chain(MYL)gene clusters as potential key players in the development of subcutaneous fat in Angus cattle.
NETL
NSTL
万方数据
