首页|Exploration of pathogenic microorganism within the small intestine of necrotizing enterocolitis

Exploration of pathogenic microorganism within the small intestine of necrotizing enterocolitis

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Background Necrotizing enterocolitis(NEC)is the most common severe gastrointestinal emergency in neonates.We designed this study to identify the pathogenic microorganisms of NEC in the microbiota of the small intestine of neonates.Methods Using the 16S ribosomal DNA(rDNA)sequencing method,we compared and analyzed the structure and diversity of microbiotas in the intestinal feces of different groups of neonates:patients undergoing jejunostomy to treat NEC(NP group),neonates undergoing jejunostomy to treat other conditions(NN group),and neonates with NEC undergoing conserva-tive treatment(NC group).We took intestinal feces and saliva samples from patients at different time points.Results The beta diversities of the NP,NN,and NC groups were all similar.When comparing the beta diversities between different time points in the NP group,we found similar beta diversities at time points El to E3 but significant differences between the E2-E3 and E4 time points:the abundances of Klebsiella and Enterococcus(Proteobacteria)were higher at the E1-E3 time points;the abundance of Escherichia-Shigella(Proteobacteria)increased at the E2 time point,and the abundance of Klebsiella decreased significantly,whereas that of Streptococcus increased significantly at the E4 time point.Conclusions Our results suggest that the pathological changes of intestinal necrosis in the small intestine of infants with NEC are not directly caused by excessive proliferation of pathogenic bacteria in the small intestine.The sources of microbiota in the small intestine of neonates,especially in premature infants,may be affected by multiple factors.

MicrobiotaNecrotizing enterocolitisSmall intestine16S rDNA

Yan Wang、Kun Jiang、Qiao Xia、Xia Kang、Shan Wang、Ji-Hong Yu、Wen-Feng Ni、Xiao-Qin Qi、Ying-Na Zhang、Jin-Bao Han、Gang Liu、Lei Hou、Zhi-Chun Feng、Liu-Ming Huang

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Clinical Biobank Center,Medical Innovation Research Division of Chinese,PLA General Hospital,Beijing,China

Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing,China

Senior Department of Pediatrics,The Seventh Medical Center of PLA General Hospital,Beijing 100700,China

Department of Neurology,The First Medical Center,Chinese PLA General Hospital,Beijing 100853,China

Department of Emergency Center,National Center for Children's Health,Beijing Children's Hospital,Capital Medical University,Beijing 100045,China

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National Key R&D Program of ChinaHealth Bureau of the Logistics Support Department of the Military Commission

2022YFC270340021JSZ18

2024

10.1007/s12519-023-00756-0

世界儿科杂志(英文版)

世界儿科杂志(英文版)

CSTPCD
ISSN：
年,卷(期)：2024.20(2)
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