Protist Diversity in the Lhalu Wetland of Tibet Based on Metagenomic Sequencing
Lhalu wetland plays an important role in promoting the virtuous cycle of Lhasa's urban ecosys-tem and improving the quality of the urban environment.In this study,we explored the diversity and com-munity structure of protists in Lhalu wetland as well as its relationship with environment factors.Our aim was to provide data to help conserve regional biological resources and the aquatic environment,and to protect and utilize biodiversity.During August and September of 2020,environmental DNA samples were collected at 10 sites in Lhalu wetland(S1 and S2 as upstream sites;S3-S8 as wetland sites,and S9 and S10 as downstream sites).The species and community structure of protists in the Lhalu wetland were identified based on metagenomic sequencing,and water samples were collected simultaneously for deter-mination of environmental factors.Results are as follows:(1)A total of 421 operational taxonomic units(OTUs)were identified,belonging to 140 genera,105 families 84 orders,35 classes,and 21 phyla.Among them,Euglenozoa,Ciliophora and Cercozoa had the highest species richness,while Cercozoa,Bacillariophyta and Ciliophora had the highest abundance.(2)There were significant differences in the di-versity of protists in different regions of Lhalu wetland.The diversity of protists was high in the upstream sites,and the distribution among various groups was quite even.Protist biodiversity in the wetland was al-so high,especially the Cercozoa and Bacillariophyta.Protist diversity was low in the downstream sites,and the primary groups were Cercozoa and Ciliophora.(3)Protist diversity was affected by a variety of environmental factors.In general,the diversity was low at sites with high concentrations of nitrogen and phosphorus.The relationship between different protist groups and environmental factors was as follows:Euglenozoa was positively correlated with total phosphorus,total nitrogen and turbidity;Bacillariophyta was negatively correlated with total phosphorus and total nitrogen;Ciliophora was positively correlated with total phosphorus and pH;and Cercozoa was significantly negatively correlated with dissolved oxy-gen and pH.This study provides data necessary for assessing aquatic ecosystem conditions in the region.
万方数据
维普
