Genetic Structure of Harpadon nehereus Populations in the Coastal Waters of China Based on Microsatellite Markers
Population genetic structure analysis is the basis for establishing fishery management,and is important for conserving biodiversity and the scientific development and use of fishery resources.Micro-satellite DNA is abundant in eukaryotic genomes and,because it is dominant and has a high mutation rate,has become an effective genetic marker for differentiating fish populations.Harpadon nehereus is an im-portant small to medium-sized commercial fish species in the coastal water of China,and a vital food chain component of marine ecosystems.In this study,we analyzed the genetic structure and differentia-tion of seven H.nehereus populations[Lianyungang(LYG),Nantong(NT),Sanmen(SM),Quanzhou(QZ),Zhanjiang(ZJ),Beihai(BH)and Sanya(SY)]in the coastal waters of China,based on six microsat-ellite markers.During 2018-2020,a total of 165 wild H.nehereus specimens from the seven populations were sampled by trawl for DNA extraction and PCR amplification.A total of 111 alleles were detected,and the average allelic richness(Rs)of the seven populations ranged from 7.944 to 10.087.The ranges of the average expected heterozygosity(He)and the average observed heterozygosity(Ho)were 0.721-0.807 and 0.621-0.785,respectively.The average polymorphic information content(PIC)ranged from 0.673 to 0.768,showing a high level of genetic diversity.The genetic distance between the SY and BH populations was the lowest(0.302 2),while the highest genetic distance(0.501 9)was observed between the BH and LYG populations.The pairwise genetic differentiation index(Fst)ranged from 0.005 4 to 0.073 7,and there was significant genetic differentiation between the East and South China Sea populations(SM,QZ,ZJ,BH and SY)and the Yellow Sea populations(LYG and NT).The UPGMA tree constructed based on Nei's standard genetic distance also shows that the Yellow Sea populations(LYG and NT)were topologi-cally independent of the other populations.Regardless of whether the populations were divided into one or two gene pools for AMOVA analysis,the results show that most of the genetic variation came from individuals within populations.3D-FCA analysis and structure analysis suggest that the H.nehereus population in coastal China should be divided into two free mating groups and treated as different units for fishery management.
万方数据
维普
