The six kinds of fertilizer system are as follows. N （N）, organic manure （OM）, mixture of N, P and K （NPK）, organic manure combined with N, P and K （OMNPK）, and straw combined with N, P and K （RSDNPK）. The fertilizer of each treatment was applied in the soil at different growth stages of maize. The experiment plots are located at the slope land of typical purple soil area in Middle Sichuan. The activities of soil nitrate reductase, nitrite reductase and hydroxylamine reductase at the jointing stage, the filling stage and the maturity stage of maize were determined. The results showed that the activities of nitrate reductase, nitrite reductase, and hydroxylamine reductase increased in the treatments of N, OM, NPK, OMNPK and RSDNPK, and the best treatments for enzymatic activity were RSDNPK and OMNPK. It suggested that RSDNPK and OMNPK could have better advantages to improve the activities of nitrate reductase, nitrite reductase and hydroxylamine reductase at the slope land of purple soil area in Middle Sichuan, and enhance the as similation rate of N by crops.
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