The activity of four nicotinic receptor agonists against Bursaphelenchus xylophilus
[Aim]To investigate the insecticidal effect of four nicotine receptor agonists against pine wood nematode,Bursapheren-chus xylophilus.[Method]The nematicidal activity was measured on pine branches of Pinus massoniana grown in hydroponics.The effects of nicotinic receptor agonists in aqueous solution were examined on the activities of adenosine triphosphate(ATP),catalase(CAT),acetylcholinesterase(AChE),and superoxide dismutase(SOD)in B.xylophilus.[Result]Levamisole hydrochloride(LH),tetraimidazole hydrochloride(TH),nicotine(N),and thiophene pyrimidine tartrate(TPT)had significant toxic activity a-gainst B.xylophilus.The LC50 values of the four drugs were 0.09,0.31,0.08,and 0.14 mg·mL-1,respectively,when immersed in water for 48 h.The results of the hydroponic experiments after 12 d showed that the pine branches in the LH,TH,N,and CK treat-ment groups did not suffer from the disease(index-0).Some branches and leaves in the TPT treatment group turned yellow,with a disease index of 31％.The branches and leaves of"CK+nematode"pine completely turned yellow and wilted,with a disease index of 100％.ATP,CAT,AChE,and SOD activities in B.xylophilus treated with nicotinic receptor agonists(LH,TH,N,and TPT)for 48 h were lower than those in the control group(P＜0.05).[Conclusion]In conclusion,nicotinic receptor agonists(LH,TH,N,and TPT)have potential as good biological controls against B.xylophilus,and have the prospect of further in-depth research,development,and utilization.
万方数据
维普
