The mitochondrial genome and phylogenetic analysis of Bruchus rufimanus(Boheman,1833)
[Aim]The Bruchus rufimanus is an important agricultural insect pest.In this study,we sequenced and analyzed the mi-tochondrial genome of B.rufimanus,and it will contribute to increasing our knowledge of the mitochondrial genome and the phyloge-ny of"Sagrinae"clade group,Bruchinae and B.rufimanus.[Method]In phylogenetic analyses,we selected 22 exemplars of"Sagr-inae"clade group(Bruchinae,Sagrinae,Donaciinae and Criocerinae)as ingroups.Two species of Eumolpinae(Basilepta fulvipes and Basilepta melanopus)were used as outgroups.The phylogenetic analyses were conducted using maximum likelihood and Bayes-ian inference methods.[Result]The mitochondrial genome of B.rufimanus is a circular molecule of 16586 bp in length(GenBank accession No.OP650255),which contains 13 protein-coding genes,two ribosomal RNA genes,22 transfer RNA genes and a non-coding control region.All tRNA genes can be folded into typical cloverleaf structure,with the exception of the trnS1,which lacks the DHU arm.Furthermore,the anticodon of trnS1 gene is not the common GCU,but rather the UCU.[Conclusion]This is the first re-port of a complete mitochondrial genome of B.rufimanus.Both phylogenetic inference methods produced a similar tree topological structure:the subfamily Bruchinae,Sagrinae,Donaciinae and Criocerinae were recovered the monophyletic groups.The subfamily Bruchinae were cluster to(Donaciinae+Criocerinae)a clade.The B.rufimanus was sister to Callosobruchus maculatus.
万方数据
维普
