Targeted inhibition of Mikania micrantha root gene MmEXPA4 by double-stranded RNA
[Aim]Based on the biological characteristics of stronger rooting ability of each internode in Mikania micrantha,a bio-control technology that inhibits the expression of genes related to root growth by RNAi was developed,providing a foundation for fur-ther development of targeted control technology of M.micrantha.[Method]A key gene involved in the root development of M.mi-crantha(MmEXPA4)was identified by the homologous comparison method,and the double-stranded RNA(dsMmEXPA4)was fur-ther synthesized in vitro.Then,dsMmEXPA4 was injected into M.micrantha roots to investigate its effect on root growth.[Result]The number of adventitious roots and their fresh weight,and the length of the longest adventitious root under the dsMmEXPA4 treat-ment after 30 days were significantly reduced compared to those under the control condition.In addition,the gene expression of MmEXPA4 was significantly downregulated at days 4 and 5 post-injection.The silencing efficiency of dsMmEXPA4 was 43％on day 4 and 52％on day 5.Based on the Fluorescent Stereo Microscope Leica M165 FC images,it was found that fluorescently labeled dsMmEXPA4 could be detected through a strong green fluorescence signal in the root tissue.[Conclusion]The MmEXPA4 gene could be used as a potential target to inhibit the root development and rapid growth of M.micrantha,providing a theoretical basis for further development of biological control technology through RNA interference.
Mikania micranthadouble-stranded RNAinvasion of weedsMmEXPA4inhibit plant growth
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