Effects of volatiles from different parts of Pinus massoniana on antennal potential and behavioral responses of Monochamus alternatus
[Aim]This study aims to investigate the volatile materials from different parts of healthy Pinus massoniana,analyze the differences in the types and contents of volatiles in different parts of the plants,and evaluate the antennal potential and olfactory re-sponses of adult Monochamus alternatus to these volatiles.[Method]The static headspace method and gas chromatography-mass spectrometry were used to analyze the volatile compositions of six parts of pine:needles,1 cm branches,5 cm branches,20 cm trunks,30 cm trunks,and pine oil,and measure the antennal potential and behavioral responses of male and female adult M.alter-natus to the volatiles.[Result]In the volatile extracts from the six parts,36 different volatile compounds were detected.These parts displayed similar types of volatiles,but there were significant differences in the relative contents among them.Terpenes,including laurin,pinene,L-beta-pinene,camphene,etc.,were the predominant volatile compounds.Electroantennogram experiment showed that the 5 cm branches volatile extract had the highest value for female adults of M.alternatus,and the pine oil volatile extract had the highest value for male adults.The behavioral response experiment showed that the volatiles of each part had a certain attractive effect on the adults of M.alternatus.Among them,the female adults exhibited the strongest attraction to the volatile extracts of 5 cm branches,and the male adults exhibited the strongest attraction to the volatile extracts of 30 cm trunks and pine oil.[Conclusion]Under healthy conditions,the plant-derived attractants made of terpene volatiles in 5 cm branches,30 cm trunks,and pine oil of P.massoniana demonstrated better attraction to the M.alternatus adults.This study will provide a reference for improving the effect of existing attractants and provide a scientific basis for the prevention and control of M.alternatus pest.
万方数据
维普
