A novel method for identifying SARS-CoV-2 infection mutants via an epitope-specific CD8+T cell test

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外文摘要：Since the outbreak of the coronavirus disease 2019(COVID-19)epidemic in 2019,the public health system has faced enormous challenges.Tracking the individuals who test positive for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is a key step for interrupting chains of transmission of SARS-CoV-2 and reducing COVID-19-associated mortality.With the increasing of asymptomatic infections,it is difficult to track asymp-tomatic infections through epidemiological surveys and virus whole-genome sequencing.However,due to the cross-reactivity of neutralizing antibodies produced by multiple virus subtypes,neutralizing antibody detection cannot be used to determine whether an individual has a history of infection with a specific subtype of SARS-CoV-2.We recruited 4 human leukocyte antigen A2(HLA-A2)infections,15 individuals who received three doses of inactivated vaccines,and 30 breakthrough infections after vaccination and discussed a case-tracking approach to detect epitope-specific CD8+T cells in the peripheral blood of close contacts,including accurate HLA typing based on ribonucleic acid(RNA)-sequencing and flow cytometry data and the comparison and characterization of SARS-CoV-2 HLA-A2 and HLA-A24 epitope-specific CD8+T cells.From individuals who received three doses of inactivated vaccine,we observed that the CD8+T cell specificity for ancestral epi-topes was significantly higher than for mutated epitopes,and the fold change of CD8+T cells corresponding to mutated epitopes relative to ancestral epitopes was less than 1.The enzyme-linked immunospot(ELISpot)results further validate this result.This study forms a"method for understanding the infection history of SARS-CoV-2 subtypes based on the proportion of epitope-specific CD8+T cells in the peripheral blood of sub-jects",covering up to 46％of the population,including HLA-A2+and HLA-A24+donors,providing a novel method for SARS-CoV-2 infected case tracing.

外文关键词：

Asymptomatic coronavirus disease 2019(COVID-19)EpitopesSpecific CD8+T cellELISpot

作者：

Congling Qiu、Bo Peng、Chanchan Xiao、Pengfei Chen、Lipeng Mao、Xiaolu Shi、Zhen Zhang、Ziquan Lv、Qiuying Lv、Xiaomin Zhang、Jiaxin Li、Yanhao Huang、Qinghua Hu、Guobing Chen、Xuan Zou、Xiaofeng Liang

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作者单位：

Department of Public Health and Preventive Medicine,School of Medicine,Jinan University,Kangtai Biological Vaccine Industry Research Institute/Disease Prevention and Control Institute of Jinan University,Guangzhou 510632,China

Shenzhen Center for Disease Control and Prevention,Shenzhen 518020,China

Department of Microbiology and Immunology,Institute of Geriatric Immunology,School of Medicine,Jinan University,Guangzhou 510632,China

Key Laboratory of Viral Pathogenesis & Infection Prevention and Control(Jinan University),Ministry of Education,Guangzhou 510632,China

The Sixth Affiliated Hospital,Jinan University,Guangzhou 510632,China

Department of Systems Biomedical Sciences,School of Medicine,Jinan University,Guangzhou 510632,China

Microbiology Laboratory,Shenzhen Center for Disease Control and Prevention,Shenzhen 518020,China

Department of Communicable Diseases Control and Prevention,Shenzhen Center for Disease Control and Prevention,Shenzhen 518020,China

Central Laboratory,Shenzhen Center for Disease Control and Prevention,Shenzhen 518020,China

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基金：

Key Project of Shenzhen Science and Technology Innovation CommissionNatural Science Foundation of ChinaR&D Program of Guangzhou LaboratorySanming Project of Medicine in ShenzhenGuangDong Basic and Applied Basic Research FoundationOpen Project Fund of Guangdong Provincial People's HospitalNational Natural Science Foundation of ChinaFunding by Science and Technology Projects in GuangzhouGuangdong Basic andApplied Basic Research Foundationfellowship of China Postdoctoral Science Foundationfellowship of China Postdoctoral Science FoundationNational Key Research and Development PlanNational Key Research and Development PlanFundamental Research Funds for the Central Q4 Universities

项目编号：

JCYJ2021032411541103092169102SRPG22-006SZSM2022110232022B1515120043YKY-KF20220881902097SL2023A04J011602023A15151401172023TQ01362023M7413792023YFE01187002021YFC230160421623406

出版年：

2024

DOI：

10.1016/j.bsheal.2024.03.005

生物安全与健康 (英文)

中华医学会

生物安全与健康 (英文)

CSTPCD

ISSN：2096-6962

年,卷(期)：2024.6(3)