首页|一种崂山百合的离体快速繁殖技术

一种崂山百合的离体快速繁殖技术

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以崂山百合的鳞茎为外植体,通过植物组织培养技术成功获得了再生植株,建立了该植物的快速无性繁殖体系。整个组织培养过程分为芽的诱导、根的诱导和移栽三个阶段。适合芽诱导的最佳外植体是:鳞茎基部,最适培养基是:1/2 MS+ 0。5 mg/LNAA +0。8 mg/L KT+3%蔗糖。用于生根的最佳培养基是:1/2 MS+ 0。4 mg/L IBA+3%蔗糖。最适培养温度为25℃。不同基质对组培苗移栽的成活率有一定影响,其中在珍珠岩上的移栽成活率为98%。
A rapid propagation method for Lilium tsingtauense Gilg
Different parts ot bulb tram Liuum tsingtauense (jug were used as explains to regenerate seedlings by tissue culture., and a rapid propagation method was established. The culture process could be constituted of three stages, which were induction of buds, induction of roots and transplant. The optimal medium for induction of buds was 1/2 MS + 0.5 mg/L NAA + 0.8 mg/L KT + 3 %sucrose, while the optimal medium for induction of roots was 1/2 MS + 0.4 mg/L IBA + 3 %sucrose. The best culturing temperature was 25 X^. Different transplant mediums had some effects on the survival rate of regenerated seedlings, and the survival rate of regenerated seedlings on perlite was 98%.

Lilium tsingtauense GilgTissue culturePropagation

韩怡、任强、郭道森、刘毓、李荣贵

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青岛大学生物系 山东青岛266071

山东师范大学生命科学学院 山东济南250014

济南市花卉苗木开发中心 山东济南250103

崂山百合 组织培养 快速繁殖

济科合字山东省科技攻关项目

2008D070612010GG0020937

2011

现代生物医学进展
黑龙江省森工总医院 哈尔滨医科大学附属第四医院

现代生物医学进展

CSTPCD
影响因子:0.755
ISSN:1673-6273
年,卷(期):2011.11(18)
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