Fungi polysaccharides have become a focal point in many scientific fields as they are important biological activity and healthy function.This review discusses biological activity of fungi polysaccharides,including antitumor,antiviral,blood pressure lowering,hyperlipidemia lowing,hpyerglycemic,antioxidant,anti-fatigue,anti-radiation and so on,and discusses extraction methods,including water extraction method,acid extraction,alkaline solution extraction,enzymatic extraction,microwave-assisted extraction and so on,and purification methods,including membrane separation technology and chromatography.Finally,the development of fungi polysaccharides are prospected.