Expression Changes and Meaning of TREK-2 Potassium Ion Channel in Hippocampus in Pilocarpin-induced Epilepsy Model
Objective:To investigate the expression change of K2P channel TREK-2 in hippocampus with pilocarpin-induced epilepsy,and to explore the mechanism and siginificance of epilepsy in which TREK-2 was involved.Methods:The epilepsy model was conducted by celiac injection of lithium-pilocarpine with adult male Sprague-Dawley rats,the hippocampus tissue was collected respectively at 6 h,1 d,3 d,1 w,2 w,4 w and 8 w after the status epilepticus (SE),and the expression changes of TREK-2 in hippocampus was detected at different time points with Western-Blot.The expression of TREK-2 was down-regulated in hippocampus with TREK-2 siRNA,and the effect on status epilepticus (SE) was further observed.Results:Compared to control group,the expression of TREK-2 began to decrease at 3 d (P ＜ 0.05),decreased significantly at 1 w,2 w,4 w,(P＜ 0.01),and remained a very low level at 8 w (P ＜ 0.001).After the expression of TREK-2 declined,the latent period of epilepsy decreased significantly,and the seizure frequency of SE significantly increased within 1 h above magnitude 5.Conclusion:The decreased expression of TREK-2 potassium ion channel in hippocampus in pilocarpin-induced epilepsy model and the down-regulated TREK-2 which aggravated the status epilepticus indicated that TREK-2 participated in the ocurrence and development of epilepsy.
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