Altitude Distribution of Soil Microbial Function Diversity in Alpine Meadow in Sanjiangyuan Region, Qinghai Province
Method of spatial variation instead of temporal variation was used in the study,six sites,which from 3220 m (S1) to 4790 m (S6),were established along every 200-300 m altitude in the alpine meadow in Qinghai Sanjingyuan National Reserve.Biolog Eco-Plates method was used to explore the distribution patterns of functional diversity of carbon source utilization along an elevational gradient,trying to analyze the effect of altitude changes on the patterns and its response.The results showed that,with the increase of altitude,microbial functional diversity indexes were in a trend of decreasing first and then rising,which was similar to the variation of AWCD.Microbial metabolic structure at different altitudes presented certain degree differences detected by Detrended correspondence analysis.The utilization degree of six carbon sources were different,and the polymers was the dominant carbon source.Canonical correspondence analysis and partial Mantel test indicated that altitude,phosphorus and available phosphorus content,but not plant diversity,significantly correlated with function diversity distribution pattern in Qinghai Sanjingyuan National Reserve.Understanding carbon utilization degree at different altitude can provide fundamental data to reveal microbial life process in Sanjingyuan.