Objective: To explore the effect of insulin on the serum and bone OPG (osteoprotegerin) and RANKL (OPG receptor activator nuclear factor-kB) expressions in the type 2 diabetic rats with osteoporosis. Methods: A rat model of type 2 diabetes with osteoporosis was established by high-fat diet and 4％ fructose water for 4 weeks, and then the rats were treated with streptozocin. The expression of serum and bone OPG and RANKL between control and model group were analyzed with ELISA, bone density was detected by bone mineral density analyzer and the level of blood glucose were analyzed by blood glucose meter. Results: The expression of OPG, blood glucose, glycosylated hemoglobin,bone density of serum and bone in model group were significantly lower than those control group(P＜0.05), while the expression of RANKL was significantly higher than that of the control group (P＜0.05), and insulin can increase the expression of OPG in serum and bone of model group, and decrease the expression of RANKL in serum and bone in model group (P＜0.05). Conclusions: Insulin can decrease the expression of RANKL and increase the expression of OPG in serum and bone in type 2diabetic rats with osteoporosis.
Type 2 diabetic rats with osteoporosisInsulinOPGRANKL
NETL
NSTL
万方数据
维普
