目的:探讨巨噬细胞膜仿生的纳米铁颗粒(Fe3O4NCs@MM)对多形性胶质母细胞瘤MRI成像的研究。方法:制备巨噬细胞膜仿生的纳米铁颗粒Fe3O4NCs@MM，利用动态光散射(Dynamic Light Scattering，DLS)和透射电子显微镜(Transmission Electron Microscope，TEM)对其水合动力学粒径、表面电势和形态进行表征。采用SDS-聚丙烯酰胺凝胶电泳(sodium dodecyl sul-phate-polyacrylamide gel electrophoresis，SDS-PAGE)评价巨噬细胞膜的完整包覆;紫外可见光谱测定巨噬细胞膜仿生的纳米铁颗粒抗蛋白吸附能力。通过MRI成像系统，分析了含不同浓度的Fe元素(0。1-1。6mM)的Fe3O4NCs@MM在GSH存在或不存在时的T1弛豫效应。采用细胞增殖-毒性实验(Cell CountingKit-8，CCK-8)，测定巨噬细胞膜仿生纳米铁颗粒处理肿瘤细胞24h后的细胞活性。尾静脉注射巨噬细胞膜仿生纳米铁颗粒至原位胶质母细胞瘤模型中，观察成像效果。结果:巨噬细胞膜仿生的纳米铁颗粒Fe3O4NCs@MM的水合动力学粒径和表面电势分别为286。5±7。6 nm和-20。7±3。5 mV，且在水溶液中分布均匀，具有较好的单分散性。包覆巨噬细胞膜的纳米铁颗粒具备抗蛋白吸附的能力。MRI成像显示，制备的巨噬细胞膜仿生的纳米铁颗粒Fe3O4 NCs@MM为GSH响应型MRI对比剂，具有较好的T1-加权磁共振成像效果，在尾静脉注射巨噬细胞膜的纳米铁颗粒0。5 h后，肿瘤部位的信号可见增强。结论:巨噬细胞膜仿生的纳米铁颗粒Fe3O4NCs@MM可实现多形性胶质母细胞瘤的MRI成像。
Experimental Study of Nanoparticles with Biomimetic Macrophage Membrane for MRI Imaging of Glioblastoma
Objective:To investigate the MRI imaging of glioblastoma multiforme with nanoparticles biomimetic to macrophage cell membrane(Fe3O4 NCs@MM).Methods:The nanoparticles with macrophage cell membrane(Fe3O4 NCs@MM)were prepared and characterized by Dynamic Light Scattering(DLS)and Transmission Electron Microscope(TEM).Sodium dodecyl sulphate-polyacry-lamide gel electrophoresis(SDS-PAGE)was used to evaluate the complete coating of extracted macrophage cell membranes.The an-ti-protein adsorption ability of nanoparticles with macrophage cell membrane was determined by UV-VIS spectroscopy.The T,relaxation effects of Fe3O4NCs@MM with Fe at different concentrations(0.1-1.6 mM)in the presence or absence of GSH were analyzed by MRI.Cell Counting Kit-8(CCK-8)was used to determine the cell activity of tumor cells treated with macrophage cell membrane bionic nanoparticles for 24 h.Glioblastoma-bearing mice were injected with macrophage cell membrane biomimetic nanoparticles and scanned by MRI.Results:The hydration kinetic particle size and surface potential of macrophage cell membrane biomimetic nanoparticle(Fe3O4 NCs@MM)were 286.5±7.6 nm and-20.7±3.5 mV respectively,with good monodispersion.Nanoparticles coated with macrophage cell membrane can successfully resist protein adsorption.MRI imaging showed that the prepared macrophage membrane bionic nanopar-ticles(Fe3O4 NCs@MM)were considered as GSH-responsive MRI contrast agents for T1-weighted MRI imaging.The enhanced signal at the tumor site can be observed at 0.5 h post-injection of Fe3O4 NCs@MM.Conclusion:MRI imaging of glioblastoma multiforme can be achieved by biomimetic nanoparticles of macrophage cell membrane(Fe3O4 NCs@MM).
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