Analysis of the Relationship between the Expression of Ring Finger Protein 2 and Ring Finger Protein 6 in Esophageal Carcinoma Tissues and Epithelial-Mesenchymal Transformation and Prognosis
Objective:To investigate the relationship between the expression of ring finger protein 2(RNF2)and ring finger protein 6(RNF6)in esophageal carcinoma tissues and the epithelial-mesenchymal transformation(EMT)and prognosis.Methods:162 patients with esophageal carcinoma admitted to Affiliated Hospital of Guangdong Medical University from February 2017 to February 2020 were selected,and the surgically removed carcinoma tissues and adjacent cancerous tissues were taken.Immunohistochemical staining was used to detect the expression of RNF2,RNF6,and EMT marker proteins[E-cadherin,N-cadherin Slug,and Snail].The relationship be-tween RNF2,RNF6,and EMT marker protein were analyzed by Spearman correlation analysis;the differences in RNF2 and RNF6 expression in different clinical pathological features of esophageal cancer tissue were analyzed;Kaplan-Meier survival curve was used to analyze the relationship between the expression of RNF2 and RNF6 and the prognosis of patients with esophageal carcinoma;Multivari-ate Cox regression was used to analyze the prognostic factors of patients with esophageal carcinoma.Results:The positive expression rates of RNF2,RNF6,N-cadherin,Slug and Snail in esophageal carcinoma tissue were higher than those in adjacent cancerous tissues,the positive expression rate of E-cadherin protein was lower than that in adjacent canceroustissues(P＜0.05).The positive expression rates of RNF2 and RNF6 proteins in esophageal carcinoma tissues were positively correlated with the positive expression rates of N-cad-herin,Slug and Snail proteins,and negatively correlated with the positive expression rate of E-cadherin protein(P＜0.05).Poorly differen-tiated,TNM stage ⅢA,tumor diameter ≥2 cm,lymph node metastasis in esophageal cancer tissue with higher positive expression rates of RNF2 and RNF6 proteins than without lymph node metastasis,tumor diameter＜2 cm,medium to high differentiated,TNM stage Ⅰ-Ⅱesophageal cancer tissue(P＜0.05).The 3-year OS rate in patients with positive expression of RNF2 was 47.17％,which was lower than 59.26％in patients with negative expression of RNF2(P＜0.05),the 3-year OS rate in patients with positive expression of RNF6 was 47.06％,which was lower than 63.41％in patients with negative expression of RNF6(P＜0.05).Multivariate Cox regression analysis showed that TNM stage ⅢA,lymph node metastasis,positive expression of RNF2 and positive expression of RNF6 were risk factors for prognosis of patients with esophageal carcinoma(P＜0.05).Conclusion:The positive expression rates of RNF2 and RNF6 increased in esophageal carcinoma tissues,and they are related to tumor diameter,differentiation degree,TNM stage,lymph node metastasis,and low survival rate.RNF2 and RNF6 may participate in the malignant progression of esophageal carcinoma through EMT.
Esophageal carcinomaRing finger protein 2Ring finger protein 6Clinical pathological featuresEpithelial-mesenchy-mal transformationPrognosis
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