Effect of Qidantongmai Tablet on lncRNA XIST Expression in Rats with Adriamycin Induced Dilated Cardiomyopathy
Objective:To investigate the therapeutic effect of Qidantongmai tablet(QDTM)on adriamycin-induced dilated cardiomyopathy(DCM)in rats and its effect on the expression of long non-coding RNA(lncRNA)X-inactive specific transcript(XIST).Methods:Rats were divided into Con group(n=12),DCM group(n=13),L-QDTM group(n=13),M-QDTM group(n=13),and H-QDTM group(n=13).Rats in Con group rats were normal control,and rats in other groups were doxorubicin-induced dilated cardiomyopathy model rats.Rats in Con group and DCM group were given normal saline,rats in L-QDTM group,M-QDTM group and H-QDTM group were given QDTM extractum powder of 500,1000 and 2000 mg/kg/d,respectively.The rats of each group were given the drug once a day for 4 weeks.After treatment,The cardiac function parameters,serum myocardial injury indexes and myocardium tissue oxidative stress indicators of each group were detected,respectively.Myocardial morphology,fibrosis and apoptosis were observed by hematoxylin eosin(HE),Masson tricolor and TUNEL staining.The transcription levels of XIST,collagen Ⅰ,collagen Ⅲ,TGF-β1,Bax and Bcl-2 in myocardial tissue were detected by RT-qPCR.Results:Compared with Con group,left ventricular ejection fraction(LVEF)and left ventricular short-axis shortening rate(FS)in DCM group decreased,left ventricular end-diastolic diameter(LVIDd)and left ventricular end-systolic diameter(LVIDs)increased,lactate dehydrogenase(LDH),creatine kinase(CK)and cardiac troponin Ⅰ(cTnⅠ)increased,myocardial injury was obvious,fibrosis area increased,collagen Ⅰ,collagen Ⅲ and TGF-β1 mRNA levels increased,TUNEL positive rate increased,Bax mRNA level increased,Bcl-2 mRNA level decreased,superoxide dismutase(SOD)and catalase(CAT)levels decreased,malondialdehyde(MDA)level increased,and XIST level increased(all P＜0.05).Compared with DCM group,LVEF and FS in L-QDTM group,M-QDTM group and H-QDTM group increased,LVIDd and LVIDs decreased,LDH,CK and cTnⅠ decreased,myocardial injury was alleviated,fibrosis area decreased,collagen Ⅰ,collagen Ⅲ and TGF-β1 mRNA levels decreased,TUNEL positive rate decreased,Bax mRNA level decreased,Bcl-2 mRNA level increased,SOD and CAT levels increased,MDA level decreased,and XIST level decreased(all P＜0.05).Conclusion:This study indicates that Qidantongmai tablet is effective in the treatment of doxorubicin-induced dilated cardiomyopathy in rats,and the mechanism may be related to the inhibition of lncRNA XIST.
NETL
NSTL
万方数据
维普
