Relationship between PAK4,PAK5 Protein Expression and Epithelial-mesenchymal Transition,Clinicopathological Features and Prognosis in Non-small Cell Lung Cancer
Objective:To investigate the relationship between p21-activated kinase(PAK)4,PAK5 protein expression and epithelial-mesenchymal transition(EMT),clinicopathological features and prognosis in non-small cell lung cancer(NSCLC).Methods:100 NSCLC patients who were admitted to our hospital from January 2018 to December 2019 were selected,collect surgically excised cancer tissue and adjacent tissue samples,the expressions of PAK4,PAK5 and EMT-related proteins[E-cadherin(E-Cad),N-cadherin(N-Cad)and vimentin(VIM)]in NSCLC tissues and tissues adjacent to cancer were detected by immunohistochemistry.The relationship between the expression of PAK4 and PAK5 proteins and the pathological features of NSCLC patients and the correlation with EMT-related proteins were analyzed.Patients were divided into positive/negative expression group according to the expression of PAK4 and PAK5 in NSCLC tissues.Survival curves of NSCLC patients with positive/negative expression of PAK4 and PAK5 were drawn by K-M method,and the influencing factors of death in NSCLC patients were analyzed by multivariate Cox regression analysis.Results:Compared with tissues adjacent to cancer,positive expression rates of PAK4,PAK5,N-Cad and VIM proteins in NSCLC tissues were increased,positive expression rate of E-Cad protein was decreased(P＜0.05).Two column correlation analysis showed that,PAK4 and PAK5 were negatively correlated with the positive expression rate of E-Cad protein in NSCLC tissues,there was a positive correlation between the positive expression rate of N-Cad and VIM protein(all P＜0.001).The positive expression rates of PAK4 and PAK5 protein in NSCLC patients with different differentiation degree,TNM stage and lymph node metastasis were compared,the difference was statistically significant(P＜0.05).The 3-year overall survival rate of 100 NSCLC patients was 56.00％(56/100).K-M survival curve analysis showed that,overall survival rate of PAK4 and PAK5 positive expression group was lower than that of negative expression group(P＜0.05).Multivariate Cox regression analysis showed that,poor differentiation,TNM stage ⅢA,lymph node metastasis and positive expression of PAK4 and PAK5 proteins were independent risk factors for death in NSCLC patients(P＜0.05).Conclusion:The expression of PAK4 and PAK5 proteins in NSCLC tissue increases,which is relate to EMT,differentiation,TNM stage,lymph node metastasis and prognosis,may become a new target for the diagnosis and treatment of NSCLC.
NETL
NSTL
万方数据
维普
