Study on the Relationship between PRRX1,VASH-1 and Microvessel Density,Clinicopathological Parameters and Prognosis in Non-Small Cell Lung Cancer Tissue
Objective:To investigate the relationship between paired related homeobox 1(PRRX1),vascular inhibitory protein(VASH-1)and microvessel density(MVD),clinicopathological parameters and prognosis in non-small cell lung(NSCLC)cancer tissue.Methods:Cancer tissues and paracancerous normal tissue specimens of 156 patients with NSCLC who underwent surgical resection in Liaoning Jinqiu Hospital from January 2018 to January 2020 were selected.Immunohistochemical staining method was applied,PRRX1 and VASH-1 positive expression rates in cancer tissues and adjacent tissues were detected,and MVD count was performed.The MVD counts in PRRX1 positive expression group/negative expression group and VASH-1 positive expression group/negative expression group were compared.The relationship between PRRX1,VASH-1 and pathological parameters of patients with NSCLC were analyzed.Relationship between PRRX1 and VASH-1 positive/negative expression and the prognosis of patients with NSCLC were analyzed by Kaplan-Meier survival curve after 3 years of follow-up.Results:Compared with adjacent tissues,PRRX1 positive expression rate was decreased in cancer tissues of patients with NSCLC,and VASH-1 positive expression rate was increased(P＜0.05).Compared with PRRX1 negative patients with NSCLC,MVD in cancer tissues of PRRX1 positive patients with NSCLC decreased,compared with VASH-1 negative patients,MVD in cancer tissues of VASH-1 positive patients with NSCLC increased(P＜0.05).Compared with TNMⅠ～Ⅱ stage and non-lymph node metastasis patients with NSCLC cancer tissues,PRRX1 positive expression rate in TNM ⅢA and lymph node metastasis patients with NSCLC cancer tissues was decreased,and VASH-1 positive expression rate was increased(P＜0.05).Kaplan-Meier analysis showed that,the 3-year overall survival(OS)and 3-year disease-free survival(DFS)in PRRX1 positive group were higher than those in PRRX1 negative group(P＜0.05),and the 3-year OS and 3-year DFS in VASH-1 negative group were higher than those in VASH-1 positive group(P＜0.05).Conclusion:The positive expression rate of PRRX1 in cancer tissues of patients with NSCLC is decrease,and the positive expression rate of VASH-1 is increase,which are relate to lymph node metastasis,TNM stage and poor prognosis.
NETL
NSTL
万方数据
维普
