Study on the Influence of Acupuncture Method of"Tiaoshenchangzhi"Acupuncture on the 5-HT4R and CaM-MLCK Signaling Pathway in the Colon Tissue of Rats with Parkinson's Constipation Model
Objective:To discover the effects of"Tiaoshenchangzhi"acupuncture on 5-HT4R and CaM-myosin in the colon tissue of rats with Parkinson's disease with constipation,and to examine their possible mechanisms of action.light link protein kinase(MLCK)signaling pathway in the colon tissue of rats with Parkinson's disease with restriction,and to explore their possible mechanisms of action.Methods:60 rats were classified into the blank group,the sham operation group,the modelling group,the western medicine group,the regular acupuncture group and the"Tiaoshenchangzhi"acupuncture group using a casual number table,with 10 rats in each group.The rat model of Parkinson's disease(PD)with constipation was made by subcutaneous injection of rotenone into the neck and back of model group,routine acupuncture group and Tiaoshenchangzhi acupuncture group,and detected by APO induction.In the conventional acupuncture group,Tianshu,Zusanli,Shangjuxu and dance tremor control areas were selected and electroacupuncture was performed with continuous wave frequency of 15Hz and stimulation intensity of 2 mA.The needles were kept for 30 minutes,once a day,for con-secutive 4 weeks.Tiaoshenchangzhi acupuncture group:Professor Wang Shun's"TiaoshenChangzhi"thirty-nine needle method was selected,Baihui penetrating the Taiyang,Zhongwuan,Qihai,Zusanli and Taichong.Soil continuous wave was used,with the frequency of head needle 45Hz,abdominal needle 30Hz and limb needle 15Hz(To reflect the quantitative stimulation of the weight of the head needle,the weight of the abdomen needle and the light of the limbs needle of The 369 acupuncture method of Tiaoshenchangzhi)＞the stimulation intensity was about 2mA,and the needle was left for 30 min,once a day,for consecutive 4 weeks;The western medicine group received metopar and mosapride daily gavage for 4 weeks.Model group and sham operation group:gavage of 2 mL normal saline daily for 4 con-secutive weeks;Blank groups are not processed.Western blot was used to detect the expression and content of 5-HT4R,CaM and MLCK protein in the proximal colon tissues of each group.Results:Compared with the blank group,the expression of 5-HT4R,CaM and MLCK protein in the proximal colon tissue of model group were significantly decreased(P＜0.01).Compared with the model group,the expres-sion of 5-HT4R,CaM and MLCK protein in the proximal colon tissue of three treatment groups(Western medicine group,routine acupuncture group and Tiaoshenchangzhi acupuncture group)were significantly decreased Elevated(P＜0.01).Compared with conventional acupuncture group,the expression of 5-HT4R,CaM and MLCK protein in Tiaoshenchangzhi group was significantly increased(P＜0.05).Conclusion:Acupuncture can ameliorate the symptoms of constipation in the rat model of PD with constraints,and the"Tiaoshen-changzhi"acupuncture group was better than the conventional acupuncture group.The mechanism of action may be realized by adjusting the 5-HT4R protein expression and activating the CaM-MLCK signaling pathway in the rat colon tissue.
AcupunctureTiaoshenchangzhi methodParkinson's disease with constipation5-HT4RCaM-MLCK signaling pathway
万方数据
维普
