Effect of Qi-descending and Phlegm-resolving Massage on Airway Remodeling and Serum NO Level in Young Rats with Chronic Asthma
Objective:To explore the therapeutic effect of qi-descending and phlegm-resolving massage on young mice with chronic asthma,and to accumulate theoretical basis for optimizing clinical treatment of chronic asthma in children.Methods:40 SPF grade 3-week SD female mice were selected and divided into control group,model group,drug group and massage group with 10 mice in each group by random number table method.After the model group,the drug group and the massage group were modeled as chronic asthma model,the drug group was treated with dexamethasone,the control group was injected with the same amount of normal saline,the massage group was added to the drug group on the basis of qi-descending and phlegm-resolving massage treatment,and the model group did not intervene.The results of HE staining,inflammatory cell level and Th17/Treg cell ratio in Bronchoalveolar Lavage Fluid(BALF)were compared in lung tissues of all groups,and the expression levels of Treg and TH17-related genes in lung tissues were de-tected.Results:Compared with the model group,the inflammatory infiltration of peribronchial tissue and massage group is light,the structure of the alveolar wall and bronchial wall is relatively complete,a small amount of goblet cell hyperplasia in the airway,the airway wall thickness and airway smooth muscle thickness increased less,and the tissue inflammatory infiltration,the total thickness and airway smooth muscle thickness are lower than the drug group(P＜0.05);Regarding the inflammatory cell levels,the control pups had a lower total number of BALF cells and the highest number of monocytes in the leukocyte classifiication.The total number of cells in the BALF of the model group was significantly higher,and neutrophils and eosinophils were mainly included in the leukocyte classification,and the total number of leukocytes and eosinophils were significantly higher than that of the control group(P＜0.05);Compared with the model group,the total number of cells and the proportion of neutrophils and eosinophils were signifiicantly lower in the drug group and the mas-sage group,but still significantly higher than the control group,and the drug treatment group was higher than the massage group(P＜0.05);Compared with the control group,the proportion of Th 17,the proportion of Treg decreased and the ratio of Th 17/Treg increased in the BALF(P＜0.05);Compared with the model group,the proportion of Th 17 and Th 17/Treg decreased in the drug group and the massage group,and the Treg ratio was lower than the drug group,and the Treg ratio was higher than the massage group(P＜0.05);Compared with the control group,the expression levels of IL-6,IL-10,IL-23 and TGF-β mRNA in the other groups were increased,and compared with the model group,the expression levels of the drug group and the massage group were reduced,and the massage group was lower than the drug group(P＜0.05);Compared with the control group,NO and IgE levels were increased,NO and IgE levels were decreased in the drug group and the massage group compared with the model group,and the massage group was lower than the drug group(P＜0.05).Conclu-sion:Qi-descending and phlegm-resolving massage,thus improving airway remodeling and playing a therapeutic role by regulating Th17/Treg ratio,expression of related inflammatory factors and types of white blood cells,the serum levels of NO,IgE,and inflammatory response.
Chronic AsthmaQi-descending and phlegm-resolvinInflammatory responseTh17/TregNOBALF
万方数据
维普
