Study on Total Saponins of Endophytic Fungi in Actinidia Arguta Root Against Hepatocellular Carcinoma
Objective:To study the total saponins in the extract of endophytic fungus C15 from Actinidia arguta root and their ef-fects on the apoptosis of hepatoma HepG 2 and Huh-7 cells,and to explore the possible mechanism.Methods:The effects of total saponins of AARTS(Total Saponins from the roots of Actinidia arguta,AARTS)and C15TS(C15 Total Saponins,C15TS)on the cell vi-abilities of HepG 2 and Huh-7 hepatoma cells were determined by CCK-8(Cell Counting Kit-8)method;The cell migration and invasion abilities were detected by cell scratch assay and Transwell assay;Western Blot(WB)was used to detect the expression levels of three apoptosis-related proteins(Bax,Bcl-2,Caspase-3)in HepG 2 and Huh-7 hepatoma cells;Annexin V-FITC/PI staining was used to detect the apoptotic period of HepG 2 and Huh-7 cells.Results:According to cell viability tests,AARTS and C15TS inhibited the viabilities of HepG 2 and Huh-7 cells in a dose-dependent manner at concentrations ranging from 50 to 400 μg/mL.The cell scratch test showed that the concentration of AARTS and C15TS at 200 and 400 μg/mL could decreased the migration abilities of HepG 2 and Huh-7 cells.In Transwell assay,AARTS and C15TS at concentrations of 200 and 400 μg/mL inhibited the invasion abilities of HepG 2 and Huh-7 cells.In WB experiment,after incubation with 200 and 400 μg/mL AARTS and C15TS for 24 h,the expression of Bax and Caspase-3 protein increased in HepG 2 and Huh-7 cells,while the expression of Bcl-2 protein decreased.In the Annexin V-FITC/PI staining experiment,both the AARTS groups and the C15TS groups with concentrations of 200 and 400 μg/mL had the abilities to promote the apoptosis of HepG 2 and Huh-7 cells,and the apoptosis was mostly early.Conclusion:Similar to AARTS,the C15TS can also inhibit the prolifera-tion,migration and invasion of HepG 2 and Huh-7 cells,and promote cell apoptosis,which may be related to P53 signaling pathway.
万方数据
维普
