Relationship between Serum Exosomes lncRNA PCGEM1,miR-129-5p and Clinical Pathological Characteristics and Prognosis of Patients with Non-Small Cell Lung Cancer
Objective:To study the relationship between serum exosomes long non-coding ribonucleic acid(lncRNA)prostate cancer gene expression marker 1(PCGEM1),micro ribonucleic acid(miR)-129-5p and clinical pathological characteristics and prognosis of patients with non-small cell lung cancer(NSCLC).Methods:125 patients with NSCLC who were admitted to Nanjing Brain Hospital from February 2016 to January 2018 were selected as NSCLC group,70 healthy individuals who were underwent physical examinations were selected as health group during the same period.The venous blood were collected of two groups,and the serum exosomes were ex-tracted.The expression of serum exosomes lncRNA PCGEM1 and miR-129-5p were detected by the real time quantitative polymerase chain reaction(qRT-PCR).The relationship between lncRNA PCGEM1 and miR-129-5p was analysed by Pearson correlation.And the relationship between serum exosomes lncRNA PCGEM1,miR-129-5p and clinical pathological characteristics of patients with NSCLC was analyzed.5 years followed up for patients with NSCLC,the prognosis was analyzed by plotted Kaplan-Meier curve,the risk factors for poor prognosis were analyzed by multivariate Cox proportional risk regression model,and the predictive value of lncRNA PCGEM1 and miR-129-5p of predicted the NSCLC prognosis was analyzed by the receiver operating characteristic(ROC)curve.Results:The rela-tive expression level of lncRNA PCGEM1 of NSCLC group was higher than that of healthy group,while the relative expression level of miR-129-5p was lower than that of healthy group(P＜0.05).The relative expression level of serum exosomes lncRNA PCGEM1 was neg-atively correlated with the expression of miR-129-5p(r=-0.420,P＜0.05).The expression of serum exosomes lncRNA PCGEM1 and miR-129-5p were related to TNM stage,differentiation degree,and lymph node metastasis of patients(P＜0.05).The Kplan-Meier survival curve showed that the 5 years survival rate of lncRNA PCGEM1 low expression group was 69.05％higher than 35.53％ of lncRNA PCGEM1 high expression group,and the 5 years survival rate of miR-129-5p high expression group was 68.09％ higher than 33.80％of miR-129-5p low expression group.Multivariate Cox proportional risk regression showed that TNM stage Ⅲ,lymph node metastasis,high expression of lncRNA PCGEM1,and low expression of miR-129-5p were independent risk factors for poor prognosis of patients with NSCLC(P＜0.05).ROC curve showed that the area under the curve(AUC)of predicted the NSCLC prognosis by the combined detection of lncRNA PCGEM1 and miR-129-5p was 0.865,and the predictive value was higher than both separately predicted.Conclusion:The expression of serum exosomes lncRNA PCGEM1 of patients with NSCLC is upregulated,while the expression of miR-129-5p is down-regulated,the expression of both is related to TNM stage,differentiation degree,and lymph node metastasis of patients with NSCLC,and closely related to patient prognosis,which has good predictive value for poor prognosis of NSCLC.
万方数据
维普
