Effect of Enema with Kuijie Decoction on Intestinal Mucosal Barrier Function and TLR4/NF-κB Signaling Pathway in Peripheral Blood Mononuclear Cells in Patients with Ulcerative Colitis of Damp Heat with Blood Stasis Type
Objective:To observe the effect of enema with kuijie decoction on intestinal mucosal barrier function and toll-like re-ceptor 4(TLR4)/nuclear factor(NF)-κB signaling pathway in peripheral blood mononuclear cells of patients with ulcerative colitis(UC)of damp heat with blood stasis type.Methods:96 patients with active UC of damp heat with blood stasis type who were admitted to Changsha Hospital of Traditional Chinese Medicine from April 2020 to August 2022 were selected,and patients were divided into exper-imental group(conventional western medicine and enema with kuijie decoction treatment,n=48)and control group(conventional west-em medicine treatment,n=48)according to the random number table method.The chinese medicine syndrome score,modified Baron en-doscopy score,inflammatory bowel disease questionnaire(IB-DQ),serum intestinal mucosal barrier function indexes[D-lactic acid(D-LA),diamine oxidase(DAO),endotoxin(LPS)]and TLR4/NF-κB signaling pathway related indexes were observed in two groups.Results:After treatment,the scores of general fever,diarrhea mucopurulent bloody stool,tenesmus,scanty dark urine,limb burnout,chest and flank distension and pain,abdominal pain and abdominal distension,anal burning and dull complexion in two groups decreased,and those in experimental group were lower than control group at the same time point(P＜0.05).After treatment,the modified Baron en-doscopy scores in two groups decreased,and experimental group was lower than control group at the same time point(P＜0.05),after treatment,the IB-DQ scores in two groups increased,and experimental group was higher than control group(P＜0.05).After treatment,D-LA,DAO and LPS in two groups decreased,and those in experimental group were lower than control group at the same time point(P＜0.05).After treatment,TLR4 mRNA and NF-κB mRNA in two groups decreased,and those in experimental group were lower than control group at the same time point(P＜0.05).Conclusion:Enema with kuijie decoction treatment can effectively improve the clinical symptoms of patients with damp heat with blood stasis type UC,reduce traditional chinese medicine syndrome scores,improve intestinal mucosal barrier function,and regulate TLR4/NF-κB signaling pathway in peripheral blood monocytes.
万方数据
维普
