The Effect of Huan Kui Le on HP A Axis and 5-HT Signal Pathway Related Molecules in UC Mice
Objective:To investigate the effect of traditional Chinese medicine(TCM)Huan Kui Le(HKL)on key molecules of hypothalamic-pituitary-adrenal(HPA)axis and 5-HT signaling pathway in ulcerative colitis(UC)mice.Methods:C57BL/6 mice(n=38)were randomized into HKL intervention group(HKL group),5-aminosalicylic acid intervention group(5-ASA group),UC model group(UC group)and normal group.The first three groups of mice were intervened with 2％dextran sodium sulfate(DSS)for 7 days to build a mouse UC model.At the same time HKL,5-ASA and distilled water were gavaged for 14 days from the first day of DSS intervention,respectively.Observed the general status and faecal traits of each mouse.Serum and colon tissue were collected after 14 days.HE staining histopathological tests were used to evaluate colon tissue integrity,injury degree,and healing of each mouse.The protein expression levels of Adrenocorticotrophic hormone(ACTH),Corticotropin releasing hormone(CRH),Cortisol(CORT),5-Hydroxytryptamine(5-HT),Tryptophane(TRP),and Kynurenine(KYN)in serum were measured by enzyme-linked immunosorbent assay(ELISA).The mRNA levels of SERT and TPH1 were determined by RT-qPCR.Results:The colonic tissue injury of mice treated with HKL and Chinese medicine re-covered.Compared to the Normal group,the serum content of ACTH,KYN,TRP and 5-HT was significantly higher(P＜0.05 or P＜0.01)and CRH content was significantly lower in UC group(P＜0.001).Compared to the UC group,the serum content of CORT,ACTH,KYN and TRP was significantly decreased in HKL group(P＜0.05 or P＜0.01),while the CRH content was significantly increased(P＜0.01).The mRNA expression level of TPH1 in the colon tissue of the UC group was significantly higher than Normal group(P＜0.01).Compared to the UC group,the mRNA expression level of TPH-1 was significantly decreased(P＜0.05),and SERT was significantly increased(P＜0.01)in HKL group.Conclusion:In UC mice,HPA axis is hyperactivated,5-HT signaling pathway is abnormal,and intestinal inflammation is obvious.The HKL has regulatory effects on the excessive activation of HPA axis,and has impacts on the expression of molecules related to 5-HT signaling pathway.
Ulcerative colitisHPA axis5-HT signal pathwayHuan Kui Le
NETL
NSTL
万方数据
