Anti-inflammatory Effect of Camel's Fur on Joint Function,Joint Synovial Tissue NLRP3,ASC,Caspase-1 in Rats with Rheumatoid Arthritis
Objective:To investigate the anti-inflammatory effects of camel's bark on joint function,joint synovial tissue NLRP3,ASC,Caspase-1 in rats with rheumatoid arthritis.Methods:Collagen induced arthritis model was adopted.The rats were divided into control group(sham operation group),model group,camelback group,Voltaline group and methotrexate group.Measurement of joint circumference and pain;Joint synovial tissue HE staining;The apoptosis of synovial tissue was observed by TUNEL staining.The ex-pressions of NLRP3,ASC,Caspase-1 in synovial tissues of rats were detected by immunohistochemistry.Results:Compared with blank group,the swelling degree and joint pain index of left and right foot joint in model group,methotrexate group,camelback group and voltaline group were higher(P＜0.05).Compared with model group,the left and right foot joint swelling degree and joint pain index in methotrexate group,camphorus group and voltaalin group were lower(P＜0.05),and the left and right foot joint swelling degree and joint pain index in camphorus group were significantly lower than those in methotrexate group and Voltaalin group.Compared with blank group,NLRP3,ASC and Caspase-1 in model group,methotrexate group,camelback group and Voltaalin group were higher(P＜0.05).Compared with model group,NLRP3,ASC and Caspase-1 were lower in Methotrexate group,Camphorus group and Voltaalin group(P＜0.05),and NLRP3,ASC and Caspase-1 in camphorus group were significantly lower than those in methotrexate group and Voltaalin group.Compared with blank group,the apoptosis rate of model group,methotrexate group,camelaria group and voltaline group was higher(P＜0.05).Compared with model group,the apoptosis rate of methotrexate group,camel peng group and voltaalin group was signif-icantly lower(P＜0.05),and the apoptosis rate of camel peng group was significantly lower than that of Methotrexate group and Voltaalin group.Compared with the model group,the articular cartilage surface of Methotrexate group,Voltaline group and camphor group were smooth,and the degree of synovial tissue hyperplasia and inflammatory cell infiltration were reduced(P＜0.05).Conclusion:Camel hair can inhibit the inflammatory response of synovial tissue by regulating the expression of NLRP3 inflammatome,so as to improve joint swelling and pain.
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