Effects of Scorpion Ointment on EGF,PDGF and their Receptors Expression in Diabetic Skin Ulcer Rat Model
Objective:To investigate the effect and the mechanism of wound healing of Scorpion ointment on the expression of EGF,PDGF and their receptors in diabetic skin ulcer rat model.Methods:Sixty rats were randomly divided into control group,model group,MEBO group and scorpion ointment group,with 15 rats in each group.Diabetic skin ulcer rat model was established,7 and 14 days after intervention,wound healing rate and Levels of epidermal growth factor(EGF),platelet-derived growth factor(PDGF)and their receptors in serum were observed and compared.Results:The wound healing rates of scorpion ointment group,MEBO group and model group were compared at the same time point at 7 d and 14 d,and the difference was statistically significant(P＜0.05 or P＜0.01);there was no significant difference between scorpion ointment group and MEBO group at 7 d and 14 d at the same time point(P＞0.05).Immunohistochemical results showed that there were statistically significant differences in the expression levels of EGF between scorpion ointment group,MEBO group and model group at 7 d(P＜0.05),but there was no statistically significant difference at 14 d(P＞0.05).There was no significant difference in the expression level of EGFR between scorpion ointment group and MEBO group,model group and blank group at 7 d and 14 d at the same time point(P＞0.05).The expression level of PDGFB in scorpion ointment group,MEBO group and model group was statistically significant at the same time point on day 7 and day 14(P＜0.05).There was no significant differ-ence in the expression level of PDGFRB between scorpion ointment group and MEBO group,model group,blank group(P＞0.05),but there was statistical significance at 14 d scorpion ointment group and MEBO group compared with model group(P＜0.05 or P＜0.01).Conclusions:Scorpion ointment can affect the expression of EGF,PDGF and their receptors in diabetic skin ulcer rat model,and promote the healing of diabetic wound.
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