Study on the Mechanism of Taohong Siwu Decoction on the Expression of VEGF,VEGFR-2,Akt Gene and Protein in Brain Microvascular Endothelial Cells of Rats with Oxygen Glucose Deprivation
Objective:To investigate the effect of Taohong Siwu decoction on the expression of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(VEGFR-2),protein kinase B(Akt)gene and protein in brain microvascular endothelial cells(rBMECs)of rats with oxygen glucose deprivation(OGD)and its mechanism.Methods:The rBMECs were cultured and randomly divided into normal group,model group,Taohong Siwu decoction(0.4 mg/mL)group and Taohong Siwu decoction(0.8 mg/mL)group.In addition to the normal group,the other three groups used oxygen glucose deprivation experiment to construct OGD injury model.Taohong Siwu decoction group was added with Taohong Siwu decoction at a concentration of 0.4 mg/mL and 0.8 mg/mL 1 h before modeling,while the normal group and the model group were added with the same volume of normal saline.Real-time quantitative polymerase chain reaction(qRT-PCR)and Western blotting were used to detect the expression of VEGF,VEGFR-2 and Akt genes and proteins.Results:Compared with the normal group,the apoptosis rate of the model group was significantly increased(P＜0.05).Compared with the model group,the Taohong Siwu decoction(0.4 mg/mL)group and Taohong Siwu decoction(0.8 mg/mL)group decreased,and the Taohong Siwu decoction(0.8 mg/mL)group was the most significant(P＜0.05).Compared with the normal group,the relative mRNA and protein expression levels of Akt,VEGFR-2 and VEGF in rBMECs of the model group were significantly increased(P＜0.05).Com-pared with the model group,the Taohong Siwu decoction(0.4 mg/mL)group and Taohong Siwu decoction(0.8 mg/mL)group were sig-nificantly decreased,and the Taohong Siwu decoction(0.8 mg/mL)group was the most significant.Conclusion:Taohong Siwu decoction may inhibit the apoptosis of endothelial cells and maintain the integrity of endothelial cells by reducing the expression levels of VEGF,VEGFR-2 and Akt genes and proteins in rBMECs under OGD conditions,which may be one of the mechanisms of Taohong Siwu Decoc-tion on brain protection.
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