Analysis of the Relationship between Serum DNMT1,TIM-3 and HBV-DNA Viral Load and Pregnancy Outcome in Patients with Pregnancy Combined with Hepatitis B Virus Infection
Objective:To investigate the relationship between serum deoxyribonucleic acid methyltransferase 1(DNMT1),T cell immunoglobulin mucin-3(TIM-3)and hepatitis B virus-deoxyribonucleic acid(HBV-DNA)viral load and pregnancy outcome in patients with pregnancy combined with hepatitis B virus(HBV)infection.Methods:186 pregnant women with HBV infection who were admitted to The First Affiliated Hospital of Nanjing Medical University from January 2021 to January 2022 were selected as HBV infection group,patients were divided into positive group(56 cases)and negative group(130 cases)according to HBV-DNA viral load,patients were di-vided into poor outcome group and good outcome group according to the pregnancy outcome,another 150 healthy pregnant women who underwent pregnancy tests at The First Affiliated Hospital of Nanjing Medical University during the same period were selected as control group.Serum DNMT1 and TIM-3 levels were detected by enzyme-linked immunosorbent assay.The levels of serum DNMT1 and TIM-3 were compared between HBV infection group and control group,positive group and negative group.The influencing factors of adverse pregnancy outcomes in pregnant women with HBV infection were analyzed by Univariate and multivariate logistic regression,the predic-tive value of serum DNMT1 and TIM-3 levels for adverse pregnancy outcomes in pregnant women with HBV infection were analyzed by receiver operating characteristic(ROC)curve.Results:Compared with control group,the levels of serum DNMT1 and TIM-3 in HBV in-fection group were increased(P＜0.05).Compared with negative group,the levels of serum DNMT1 and TIM-3 in positive group were increased(P＜0.05).The incidence of adverse pregnancy outcomes in 186 pregnant women with HBV infection was 55.38％(103/186).Univariate analysis showed that adverse pregnancy outcomes was related to the gestational age of HBV infection,HBV DNA Viral load,aspartate Transaminase(AST),Alanine transaminase(ALT),DNMT1,TIM-3(P＜0.05).Multivariate Logistic regression analysis showed that,positive HBV-DNA viral load and DNMT1＞34.94 ng/mL and TIM-3＞18.96 pg/mL were independent risk factors for adverse pregnancy outcomes in pregnant women with HBV infection(P＜0.05).ROC curve analysis showed that,the area under the curve of serum DNMT1 and TIM-3 levels alone and in combination to predict adverse pregnancy outcomes in pregnant women with HBV infec-tion by detecting was 0.798,0.791 and 0.870 respectively.Conclusion:The increase of serum DNMT1 and TIM-3 levels in pregnant women with HBV infection is closely relate to the positive HBV-DNA viral load and adverse pregnancy outcomes,and combination of serum DNMT1 and TIM-3 levels has a good predictive value for pregnancy outcome in pregnant women with HBV infection.
PregnancyHepatitis B virus infectionDNMT1TIM-3HBV-DNAPregnancy outcome
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