Correlation Analysis of Serum zonulin,MCP-1 and HIF-1α with Disease Activity and Intestinal Microbiota in Patients with Crohn's Disease and Their Differential Value Analysis
Objective:To investigate the correlation of serum zonulin,monocyte chemotactic protein-1(MCP-1),hypoxia-in-ducible factor-1α(HIF-1α)with disease activity and intestinal microflora in patients with Crohn's disease(CD),and to analyze the value of zonulin,MCP-1 and HIF-1α in the identification of severe CD.Methods:162 patients with CD who were admitted to The First Affili-ated Hospital of Hunan University of Chinese Medicinel from January 2019 to January 2022 were selected.According to Crohn's disease activity index(CDAI),patients with CD were divided into severe group,moderate group and mild group,with 42,69,and 51 cases,re-spectively,another 151 physical examination volunteers during the same period were selected as the control group.The levels of serum zonulin,MCP-1 and HIF-1α in the patients with CD and control group were detected.Pearson correlation analysis was performed to analyze the correlation between zonulin,MCP-1 and HIF-1α with CDAI score and the relative abundance of intestinal flora.Receiver operating characteristic curve(ROC)was used to analyze the value of zonulin,MCP-1 and HIF-1α in identifying severe CD.Results:The levels of serum zonulin,MCP-1 and HIF-1α in the severe group,moderate group and mild group were higher than those in the control group(P＜0.05).The levels of serum zonulin,MCP-1,HIF-1α,and the relative abundance of Escherichia coli,Choleosinophilus wardeni,and Atopobium parvulum in the severe group were higher than those in the moderate group and mild group,and the moderate group was higher than the mild group(P＜0.05),and the relative abundance of Clostridium pratense in the mild group was higher than that in the moderate group,and moderate group was higher than the severe group(P＜0.05).The levels of serum zonulin,MCP-1 and HIF-1α in patients with CD were positively correlated with CDAI score,the relative abundance of Escherichia coli,Choleosinophilus wardeni and Atopobium parvulum,and negatively correlated with the relative abundance of Clostridium pratense(P＜0.05).The area under curve(AUC)of zonulin,MCP-1 and HIF-1α in the identification of severe CD was 0.856,which was higher than 0.706,0.693 and 0.747 of zonulin,MCP-1 and HIF-1α alone.Conclusion:The levels of serum zonulin,MCP-1 and HIF-1α are increased in patients with CD,and are associated with increased CD disease activity and intestinal microbiota disorder,which may be a potential biomarker for the diagnosis of severe CD.
Crohn's diseaseZonulinMCP-1HIF-1αDisease activityIntestinal floraCorrelationDifferential value
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