Relationship between the Expression of miR-145 and miR-186 and Clinical Pathological Characteristics and PI3K/Akt/mTOR Signaling Pathway in Non-Small Cell Lung Cancer Tissues
Objective:To investigate the relationship between the expression of micro ribonucleic acid(miRNA)-145,miR-186 and clinical pathological characteristics and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR)signaling pathway in non-small cell lung cancer(NSCLC)tissues.Methods:128 NSCLC patients who underwent radical surgery for lung cancer in our hospital From March 2020 to March 2023 were selected,the intraoperative cancer tissues and cancer adja-cent tissues(more than 5cm from the cancer tissues)were taken,the expression of miR-145,miR-186,PI3K mRNA,Akt mRNA and mTOR mRNA was detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The relationship between the expression of miR-145 and miR-186 and the clinical pathological characteristics of NSCLC patients was analyzed.The correlation between the expression of miR-145,miR-186 and the expression of PI3K mRNA,Akt mRNA and mTOR mRNA in NSCLC patients were analyzed by Pearson test.Results:The expression of miR-145 and miR-186 in cancer tissues was lower than that in cancer adjacent tissues(P＜0.05),the expression of miR-145 and miR-186 in NSCLC tissues with low differentiation,TNM ⅢA stage and lymph node metastasis was lower than that in NSCLC tissues with medium to high differentiation,TNM Ⅰ～Ⅱ stage and without lymph node metasta-sis(P＜0.05).The expression of PI3K mRNA,Akt mRNA and mTOR mRNA in cancer tissues was higher than that in cancer adjacent tis-sues(P＜0.05),the expression of miR-145 and miR-186 in cancer tissues was negatively correlated with the expression of PI3K mRNA,Akt mRNA and mTOR mRNA(P＜0.05).Conclusion:The expression of miR-145 and miR-186 in NSCLC tissues is down-regulate,the down-regulate of miR-145 and miR-186 expression may activate the PI3K/Akt/mTOR signaling pathway to promote the progression of NSCLC,and is associate with poor differentiation of cancer tissue,TNM ⅢA stage and lymph node metastasis in NSCLC patients.
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