Activation of the Asparaginyl Endopeptidase(AEP)and Glial Cells in a Mouse Model of Traumatic Brain Injury
Objective:There is a lack of effective treatment for traumatic brain injury(TBI),and it is needed to find new intervention targets.The asparaginyl endopeptidase(AEP)plays an important role in immune and neurological diseases,and this study observed the activation and changes of AEP in a mouse TBI model to explore the significance of AEP on brain injury and repair.Methods:Controlled cortical impact was used to create TBI damage in the right cerebral hemisphere of mice,and the lactate content and AEP activity changes in the damaged brain tissue were measured at different time points after modeling,and the activation of glial cells and the expression of AEP in TBI were observed by immunofluorescence chemical staining 3 days after TBI.Results:TBI causes lactate to accumulate in damaged brain tissue,resulting in reactive activation and proliferation of microglia and astrocytes.The upregulation and activation of AEP occur in the secondary brain injury stage of TBI,and AEP is upregulated in both microglia and astrocytes.Conclusions:AEP has the potential to play an important role in nerve injury and repair by participating in the regulation of glial cell activation triggered by TBI.
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