Effect of Phyllanthus Emblica Extract on the Biological Behavior of Lung Cancer Cell A549 by Regulating LINC01772/miR-153
Objective:To investigate the effect and mechanism of phyllanthus emblica extract on proliferation,migration and invasion of lung cancer cell A549.Methods:A549 cells were cultured in vitro and divided into control group,different doses(low,medium and high doses)phyllanthus emblica extract group,si-NC group,si-LINC01772 group,high dose phyllanthus emblica extract+pcDNA group and high dose phyllanthus emblica extract+pcDNA-LINC01772 group.Cell proliferation was detected by cell counting kit-8(CCK-8)and clone formation assay.Cell migration was detected by scratch assay.Cell invasion was detected by Transwell assay.The expression levels of E-cadherin and N-cadherin in the cells were detected by Western Blot,and the expression levels of LINC01772 and miR-153 were detected by real-time fluorescence quantitative PCR(RT-qPCR).The regulatory relationship between LINC01772 and miR-153 was verified through the dual luciferase reporter gene assay.Results:Compared with the control group,the expression of LINC01772 in A549 cells in different doses of phyllanthus emblica extract groups was decreased,and the optical density(OD value),number of clone formation,migration and invasion cells were decreased(P＜0.05),while the content of miR-153 and the expression of E-cadherin protein were increased(P＜0.05),with a dose-dependent manner(P＜0.05).LINC01772 negatively regulated miR-153 expression in A549 cells.Compared with the si-NC group,the proliferation,invasion and migration of A549 cells in the si-LINC01772 group were inhibited(P＜0.05).Compared with the high dose phyllanthus emblica extract+pcDNA group,the proliferation,invasion and migration of A549 cells in the high dose phyllanthus emblica extract+pcDNA-LINC01772 group were enhanced(P＜0.05).Conclusion:Phyllanthus emblica extract may inhibit the proliferation,migration and invasion of lung cancer cell A549 by regulating the LINC01772/miR-153 axis,which may play a role by down-regulating LINC01772 and up-regulating the expression of miR-153,and has the potential value to be developed as a drug for the treatment of lung cancer.
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