Expression of Serum LncRNA KCNQ1OT1 and miR-181 a-5p in Patients with Type 2 Diabetic Cardiomyopathy and its Correlation with Left Ventricular Function
Objective:To investigate the expression levels of serum long non-coding RNA KCNQ1OT1(LncRNA KCNQ1OT1)and microRNA-181a-5p(miR-181a-5p)in patients with type 2 diabetic cardiomyopathy(T2DCM)and their correlation with left ventric-ular function.Methods:80 T2DCM patients who were admitted to Tongji Hospital Affiliated to Tongji Medical College of Huazhong U-niversity of Science and Technology from June 2021 to June 2023 were selected as T2DCM group,80 patients with type 2 diabetes mel-litus(T2DM)were selected as T2DM group,and 80 healthy patients with simultaneous physical examination were selected as healthy control group.The expression levels of serum LncRNA KCNQ1OT1 and miR-181a-5p in three groups were detected and compared by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The correlation between serum LncRNA KCNQ1OT1,miR-181 a-5p expression and left ventricular function[early mitral valve blood flow velocity peak/left wall mitral annulus early peak ve-locity mean(E/e'),left ventricular end-diastolic diameter(LVEDD),left ventricular ejection fraction(LVEF),Tei index,left ventricular end-systolic diameter(LVESD),Peak blood flow velocity in the early mitral valve/late mitral valve blood flow velocity peak(E/A)]were analyzed by Pearson correlation analysis.Results:Body mass index(BMI),fasting blood glucose(FBG),fasting insulin(FINS),B type natriuretic peptide(BNP),creatine kinase(CK),total cholesterol(TC),glycosylated hemoglobin(HbAlc),low density lipoprotein choles-terol(LDL-C)and insulin resistance index(HOMA-IR)in T2DCM group were higher than those in healthy control group(P＜0.05).BNP,CK,TC and LDL-C in T2DCM group were higher than those in T2DM group(P＜0.05).The expression level of serum miR-181a-5p in T2DCM group was lower than that in T2DM group and healthy control group(P＜0.05).The expression level of serum LncRNA KC-NQ1OT1 in T2DCM group was higher than that in T2DM group and healthy control group(P＜0.05).LVEDD,LVESD,E/e'and Tei index in T2DCM group were higher than those in T2DM group and healthy control group(P＜0.05),LVEF and E/A were lower than those in T2DM group and healthy control group(P＜0.05).Serum LncRNA KCNQ1OT1 in T2DCM patients was positively cor-elated with LVEDD,LVESD,Ee'and Tei index(P＜0.05),and negatively correlated with E/A,LVEF and miR-181a-5p(P＜0.05).Serum miR-181a-5p was negatively correlated with LVEDD,LVESD,E/e'and Tei index(P＜0.05),and positively correlated with E/A and LVEF(P＜0.05).Conclusion:The expression level of serum LncRNA KCNQ1OT1 in T2DCM patients is increase,and the decrease of miR-181a-5p expression level may be involve in the process of left ventricular dysfunction.
Diabetic cardiomyopathyLncRNA KCNQ1OT1miR-181a-5pLeft ventricular function
NETL
NSTL
万方数据
