The Mechanism of Exosomes Derived from Adipose-derived Mesenchymal Stem Cells Promoting Endometrial Regeneration and Fertility Recovery in Rats with Intrauterine Adhesions
Objective:The purpose of this study was to identify exosomes derived from adipose-derived mesenchymal stem cells(ADSC-exo)and to explore its therapeutic potential in the rat model of intrauterine adhesion(IUA).Methods:Inguinal adipose tissue was obtained from 6-8-week-old female Sprague-Dawley rats and ADSC was isolated.Flow cytometry analysis was used to identify the ex-pression of cell surface markers(CD90,CD105,CD34 and CD45)of ADSC.The morphology of exosomes was characterized by trans-mission electron microscopy,and the expression of exosome surface marker proteins(CD63 and Alix)was analyzed by Western blotting.Hematoxylin eosin staining and Masson staining were used to analyze the morphology and fibrosis of uterine tissue.Functional tests were performed to evaluate the pregnancy rate,number of implanted embryos and pregnancy time of IUA rats.Results:ADSC-exo was cup-shaped and expressed Alix and CD63 mainly at 109.5 nm.In IUA model,ADSC-exo enhanced integrin β3,LIF and VEGF expres-sion,promoted endometrial regeneration and collagen remodeling,and maintained normal uterine structure.ADSC-exo can improve the receptivity of regenerating endometrium.Conclusion:ADSC-exo promotes endometrial regeneration and fertility recovery.The results of this study suggest that ADSC-exo is a potentially effective treatment option for severe intrauterine adhesions and infertility by local in-trauterine administration.
Mesenchymal stem cellsExosomesIntrauterine adhesionsProliferation of endometriumFertility
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