Based on DNA Methylation Technology to Explore the Mechanism of Action of Epigenetic Inheritance in Adipocytes of Nutritionally Obese Mice
Objective:The mechanism of action of nutritional obesity is more complex.The group explored the differential changes of DNA methylation in adipocytes of nutritionally obese mice from the perspective of epigenetic,to study the mechanism of obe-sity generation and progression from a new angle.Methods:SPF grade C57BL/6 mice were selected to establish a high-fat diet-induced obesity model.The mice were fed a high-fat chow for 8 weeks,and those that reached the criteria for nutritional obesity were assigned to the obese group(M).The body weights of the mice were regularly recorded.The morphology of adipose tissues was observed using HE staining.Differences in serum TC,TG,HDL-C,and LDL-C were examined using ELISA.The differences in DNA methylation of the adipocytes were analyzed using MethylRAD-seq technology to identify loci and genes with differentially expressed DNA methylation in perirenal adipose tissue cells.Results:TCompared with the normal group(N),mice in the obese group had significantly increased body weight and perirenal fat weight(P＜0.01).Additionally,the morphology of perirenal adipocytes was altered,with a significantly lower number of adipocytes in the same field of view and a significantly larger diameter(P＜0.01).The obese group also exhibited significantly higher TC levels(P＜0.01),while TG levels were significantly lower(P＜0.01).By MethylRAD-seq technology,a total of 5528 methyla-tion sites with signifiicant differences were identified in experimental rats from group N and group M.Among these sites,3003 were up-regulated and 2525 were down-regulated.Additionally,80 DNA methylation genes showed significant differences in their expression,with 44 genes being up-regulated and 36 genes being down-regulated.The GO/KEGG enrichment analysis revealed that the DNA methy-lation mechanism in the two groups of experimental rats is involved in various biological processes,molecular functions,and regulation of signaling pathways.Conclusions:A Epigenetic differential changes occur in adipocytes of nutritionally obese mice induced by a high-fat diet.Additionally,there are significant differences in gene expression and DNA methylation in perirenal adipose tissue cells of obese mice.These findings provide a new research direction for understanding the molecular and genetic mechanisms underlying the occurrence of obesity and its related diseases.
High-fat dietNutritional obesityDNA methylationMiceMechanism of action
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