Effects of Ganoderma Lucidum Phenol on Inflammatory Response and Renal Fibrosis in Rats with Chronic Kidney Disease through AGE/RAGE Signaling Pathway
Objective:To investigate the effects of ganoderma lucidum phenol on inflammatory response and renal fibrosis in rats with chronic kidney disease(CKD)through AGE/RAGE signaling pathway.Methods:Forty SD male rats were divided into model group,low-dose ganoderma lucidum phenol group,medium-dose ganoderma lucidum phenol group and high-dose ganoderma lucidum phenol group.In addition,healthy rats were selected as blank control group,with 10 rats in each group.The blank control group and the model group were injected with equal volume of normal saline,and the low,medium and high dose groups of ganoderma lucidum phenol were given 5,10,20 mg/kg ganoderma lucidum phenol respectively.After administration,renal function was detected by immunotur-bidimetry.Enzyme-linked immunosorbent assay was used to detect inflammatory factors and renal fibrosis;the body weight of the rats was weighed,the kidney weight was measured,the kidney index was calculated,and the renal lesion score was evaluated.Results:The levels of serum creatinine(Scr),urea nitrogen(BUN)and uric acid(UA)in the model group,Ganoderma lucidum phenol(low,medium and high dose)groups were higher than those in the blank control group;the levels of BUN,SCr and UA in the ganoderma lucidum phe-nol(low,medium and high dose)groups were higher than those in the model group(P＜0.05).Compared with the blank control group,the body weight of the model group and the ganoderma lucidum phenol(low,medium and high dose)group was lower,and the kidney index and lesion score were higher.Compared with the model group,the body weight of the ganoderma lucidum phenol(low,medium and high dose)groups was higher,and the kidney index and lesion score were lowe(P＜0.05).Compared with serum C-reactive protein(CRP),in-terleukin-6(IL-6),interleukin-8(IL-8)and tumor necrosis factor-α(TNF-α),the model group and ganoderma lucidum phenol(low,medium and high dose)groups were higher than the blank control group;the ganoderma lucidum phenol(low,medium and high dose)groups were lower than the model group(P＜0.05).The levels of serum hyaluronic acid(HA),type Ⅳ collagen(C-Ⅳ),laminin(LN)and type Ⅲ procollagen(PCⅢ)in the model group and the ganoderma lucidum phenol(low,medium and high dose)groups were higher than those in the blank control group.The ganoderma lucidum phenol(low,medium and high dose)groups were lower than the model group(P＜0.05).Conclusion:Ganoderma lucidum phenol can effectively inhibit inflammatory response and renal fibrosis through AGE/RAGE signaling pathway,and can also improve renal function and play a protective role in CKD rats.
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